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目的 :测定赤土茯苓中总黄酮含量。方法 :6 0 %乙醇提取液 ,三氯化铝醋酸钾显色 ,42 0nm测定紫外吸收度。结果 :12 .5- 2 5倍量 6 0 %乙醇回流提取 1h ,提取一次 ,总黄酮含量为 0 .2 6 % ;8倍量 6 0 %乙醇回流提取 1h ,提取 4次 ,总黄酮含量为 0 .4%。结论 :赤土茯苓中总黄酮含量为 0 .4%。本法简便、快速 ,可用于赤土茯苓生药及其制剂总黄酮含量测定及质量控制
Objective: To determine the content of total flavonoids in terracotta. Methods : 60% ethanol extract, aluminum trichloride, potassium acetate color, UV absorbance at 42 nm. Results: 12.5-2.5 times 60% ethanol reflux extraction 1h, extraction once, the total flavonoid content was 0.26%; 8 times 60% ethanol reflux extraction 1h, extracted 4 times, the total flavonoid content was 0.4%. Conclusion : The content of total flavonoids in terra cotta was 0.04%. This method is simple and rapid and can be used for the determination and quality control of total flavonoids in terracotta biomedicine and its preparations.