目的建立血浆中清脑宣窍方有效部位中栀子苷、人参皂苷Rg1、Rb1及三七皂苷R1的HPLC血药浓度测定方法。方法血浆样品用C18固相柱萃取,以亥茅酚苷为内标,选用Agilent Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-水二元梯度洗脱,柱温为30℃;流速为1.0mL/min;检测波长为203nm。结果各成分的最低检测浓度(S/N>3)在0.2~0.9mg/L之间,平均回收率均在90%以上,日内、日间精密度及稳定性的RSD均小于10%。结论该方法简便、准确,可作为清脑宣窍方血药浓度定量分析方法。 Objective To establish a HPLC method for determination of geniposide, ginsenoside Rg1, Rb1 and notoginsenoside R1 in the effective fraction of plasma Qingzhong Xuanxiong. Methods The plasma sample was extracted by C18 solid-phase column. The hydroquinone was used as the internal standard. The Agilent Eclipse XDB-C18 column (4.6mm×250mm, 5μm) was used. The mobile phase consisted of acetonitrile-water binary gradient elution. 30°C; flow rate 1.0 mL/min; detection wavelength 203 nm. Results The lowest concentration of each component (S/N>3) was between 0.2 and 0.9 mg/L. The average recovery rate was above 90%. The RSDs of intraday and inter-day precision and stability were all less than 10%. Conclusion This method is simple, accurate, and can be used as a quantitative analysis method for blood concentration of Qingnao Xuanxiong.