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目的 研究低强度 2 4 5 0MHz微波 (5 0mW cm2 )与 4 硝基喹啉氧化物 (4 NQO)对人淋巴细胞DNA损伤效应的联合作用。方法 采用彗星试验来检测人外周血淋巴细胞经微波与 4 NQO联合作用后的DNA损伤情况。微波与 4 NQO联合暴露方式有 3种 :微波辐射后 4 NQO染毒、微波与4 NQO同时暴露、4 NQO染毒后微波辐射。结果 微波辐射组的DNA损伤指标 (彗星形状和彗星全长 )与对照组比较 ,差异无显著性 (P >0 0 5 )。微波照射后 4 NQO染毒组中 ,5 0 ,2 5 μmol L剂量的DNA损伤指标与相应 4 NQO组比较差异均有显著性 (P <0 0 5或P <0 0 1)。微波与 4 NQO同时暴露组或 4 NQO染毒后微波照射组与相应 4 NQO组比较差异无显著性 (P >0 0 5 )。结论 低强度 2 4 5 0MHz微波不能诱发DNA损伤 ,当微波辐射先于 4 NQO暴露时 ,微波能增强 4 NQO诱发的DNA损伤效应。
Objective To investigate the combined effects of low intensity microwave (50 0 mW cm2) and 4 nitroquinoline oxide (4 NQO) on DNA damage in human lymphocytes. Methods Comet assay was used to detect the DNA damage of human peripheral blood lymphocytes combined with 4 NQO by microwave. There were 3 microwave and 4 NQO co-exposure modes: 4 NQO exposure after microwave irradiation, simultaneous exposure of microwave and 4 NQO and 4 NQO exposure to microwave radiation. Results There was no significant difference in DNA damage index (total comet shape and total comet length) between the two groups (P> 0.05). In 4 NQO-treated groups, the DNA damage index of 50 and 25 μmol L groups was significantly lower than that of the corresponding 4 NQO groups (P <0.05 or P <0.01). No significant difference was found between microwave and 4 NQO exposure group or 4 NQO exposure group and the corresponding 4 NQO group (P> 0.05). Conclusion Low intensity microwave at 2450 MHz can not induce DNA damage. When exposed to 4 NQO for microwave irradiation, microwave can enhance DNA damage induced by 4 NQO.