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目的:探讨ELISA在诊断人类免疫缺陷病毒感染中的应用价值。方法:本文的研究对象选择为我院2013年12月到2014年12月医治的人类免疫缺陷病毒感染患者100例,分别应用ELISA检测和胶体金法检测方式对患者的人类免疫缺陷病毒抗体进行检测,对比两组抗体检测的检测结果。结果:经过复查可知,ELISA检测的阳性率和特异性皆是98%,敏感性是87%;而胶体金法检测的阳性率是89%,特异性是76%,敏感性是75%。ELISA检测的阳性率、特异性以及敏感性皆比胶体金法检测检测高,数据比较有明显的差异(P<0.05),具有统计学意义。结论:ELISA在诊断人类免疫缺陷病毒感染中的应用价值高,能明确的诊断出免疫缺陷病毒感染患者,检测的阳性率、特异性以及敏感性较好,对临床的快速筛查具有积极的作用,值得应用推广。
Objective: To investigate the value of ELISA in the diagnosis of human immunodeficiency virus infection. Methods: 100 cases of human immunodeficiency virus-infected patients who were treated in our hospital from December 2013 to December 2014 were selected as the study objects, and their antibodies against human immunodeficiency virus were detected by ELISA and colloidal gold assay respectively , Comparing the two groups of antibody test results. Results: After review, the positive rate and specificity of ELISA were 98% and the sensitivity was 87%. The positive rate of colloidal gold assay was 89%, the specificity was 76% and the sensitivity was 75%. The positive rate, specificity and sensitivity of ELISA assay were higher than the colloidal gold assay test, the data were significantly different (P <0.05), with statistical significance. Conclusion: ELISA is of high value in the diagnosis of human immunodeficiency virus (HIV) infection, and can be used to diagnose patients with immunodeficiency virus infection. The positive rate, specificity and sensitivity of ELISA are positive and have a positive effect on clinical rapid screening , It is worth to promote the application.