目的预测可能的HLA-Ⅰ类限制性HBV特异性CTL表位组合在中国人群的理论免疫应答率,旨在指导乙型肝炎(HBV)多表位疫苗的研制和应用。方法利用“中国多表位疫苗设计的HLA-Ⅰ积累表型频率空间预测系统”计算HLA-A、B限制性HBV特异性CTL表位组合在中国人群中的累积表型频率(CPF),得出理论免疫应答率,并绘制CPF预测等值线图,比较结果。结果HLA-A或B单一超型组合针对中国人群的理论免疫应答率普遍偏低。HLA-A和B超型组合针对中国人群的理论免疫应答率相对较好。结论根据不同HLA组分组合设计的多表位疫苗(或DNA疫苗)在中国各地人群中的免疫效果不同,根据中国人HLA分布特点,进行疫苗理论免疫应答率预测研究,可以指导制备适合中国人群的HBV特异性CTL多表位疫苗,应用前景广阔,有重要的指导意义。 Objective To predict the theoretical immune response rate of possible HLA class I-restricted HBV-specific CTL epitope combinations in Chinese population and to guide the development and application of hepatitis B multi-epitope vaccine. Methods The cumulative phenotypic frequency (CPF) of HLA-A, B-restricted HBV-specific CTL epitope combinations in Chinese population was calculated using HLA-Ⅰ integrated phenotypic frequency spatial prediction system designed by Chinese multi-epitope vaccine. , Draw theoretical immune response rate, and draw CPF prediction contour map, compare the results. Results The theoretical immune response rate of HLA-A or B single supertype combination to Chinese population was generally low. The theoretical immune response rate of HLA-A and B supertypes to Chinese population is relatively good. Conclusions The multi-epitope vaccines (or DNA vaccines) designed according to the combination of different HLA components have different immunogenic effects among different populations in China. Based on the distribution characteristics of HLA in Chinese, the vaccine theoretical immune response rate prediction study can guide the preparation of suitable Chinese population HBV specific CTL multi-epitope vaccine, has broad application prospects and has important guiding significance.