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目的 探讨晶状体和玻璃体提取物对猪眼筋膜囊(Tenon囊)成纤维细胞增殖和胶原合成的影响及其在糖皮质激素作用下的变化。方法 体外培养猪眼Tenon囊成纤维细胞,应用噻唑蓝(MTT)比色法和逆转录聚合酶链反应(RT -PCR)法观察在晶状体和玻璃体提取物及地塞米松单独或联合作用下,Tenon囊成纤维细胞增殖和胶原合成的变化。结果 与对照细胞吸光度(A值)(0 .305±0.013)比较, 10mg/L晶状体提取物( 0. 411±0. 000 )和10mg/L玻璃体提取物( 0. 349±0 .027)作用2d即具有促进细胞生长作用,差异有统计学意义(P=0. 00);且随着晶状体提取物、玻璃体提取物浓度的增高,A值相应增加,差异均有统计学意义(P=0. 00)。Ⅰ型胶原的相对含量在晶状体提取物作用细胞内为12 290±231,在玻璃体提取物作用细胞内为10 .853±231,与对照细胞(9389±178)比较,差异均有统计学意义(P<0. 01)。地塞米松对125mg/L晶状体提取物和125mg/L玻璃体提取物作用的Tenon囊成纤维细胞增殖分别具有一定抑制作用,差异有统计学意义(P=0. 00)。地塞米松和提取物联合作用细胞内Ⅰ型胶原的相对含量与提取物单独作用细胞比较,差异均无统计学意义(P>0 .05)。结论 晶状体和玻璃体提取物均可明显刺激Tenon囊成纤维细胞增殖,促进Tenon囊成纤维细胞内胶原合成,?
Objective To investigate the effects of lens and vitreous extract on the proliferation and collagen synthesis of fibroblasts in Tenon capsule and the changes under the action of glucocorticoids. Methods Tenon’s cystic fibroblasts were cultured in vitro. MTT assay and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to observe the effects of lens and vitreous extract and dexamethasone alone or in combination. Tenon capsule fibroblast proliferation and collagen synthesis changes. Results Compared with the control cell absorbance (A value) (0.305 ± 0.013), 10 mg / L lens extract (0. 411 ± 0. 000) and 10 mg / L vitreous extract (0. 349 ± 0.277) (P = 0.00). With the increase of the concentrations of lens extract and vitreous extract, the value of A increased correspondingly (P = 0), and the difference was statistically significant 00). The relative content of type I collagen was 12 290 ± 231 in the cells of the lens extract and 10.853 ± 231 in the cells of the vitreous extractive, compared with the control cells (9389 ± 178), the difference was statistically significant ( P <0.01). Dexamethasone on 125mg / L lens extract and 125mg / L vitreous extract Tenon cystic fibroblasts proliferation had a certain inhibitory effect, the difference was statistically significant (P = 0.00). Dexamethasone and extract combined intracellular collagen type I relative content and extract alone acting cells, the difference was not statistically significant (P> 0.05). Conclusion Both lens and vitreous extract can significantly stimulate the proliferation of Tenon’s cystic fibroblasts and promote the collagen synthesis in Tenon’s cystic fibroblasts.