论文部分内容阅读
本文运用配位效应将木犀草素与金属离子Zn~(2+)和Mn~(2+)反应生成了木犀草素配合物,采用了红外、紫外、热重及元素分析对配合物进行了结构表征,同时研究了对黄嘌呤氧化酶的抑制作用,比较了木犀草素及其配合物抑制能力的大小,确定了对黄嘌呤氧化酶的抑制作用类型。结果表明,木犀草素与金属离子Zn~(2+)和Mn~(2+)发生了配位反应,形成了稳定的配合物,配合位点位于A环的5-OH及C环的4-C=O处。相比于木犀草素,木犀草素配合物对黄嘌呤氧化酶表现出更好的抑制效果,通过固定黄嘌呤底物浓度为0.6mmol/L,酶浓度对酶活力作图可知,木犀草素及其配合物对黄嘌呤氧化酶的抑制类型均属于可逆抑制,通过固定黄嘌呤氧化酶浓度为10μg/mL,用Lineweaver-Burk双倒数作图,木犀草素及其配合物对黄嘌呤氧化酶的抑制类型表现为竞争性抑制。
In this paper, luteolin was synthesized by the reaction of luteolin with Zn 2+ and Mn 2+, and the complexes were characterized by IR, UV, TG and elemental analysis Structure characterization, at the same time studied the inhibition of xanthine oxidase, comparing the inhibition of luteolin and its complex size, to determine the type of inhibition of xanthine oxidase. The results showed that luteolin interacted with the metal ions Zn 2+ and Mn 2+ and formed a stable complex. The coordination sites were located at 5-OH of ring A and 4 of ring C -C = O. Compared with luteolin, luteolin complex showed a better inhibitory effect on xanthine oxidase. By fixing the concentration of xanthine substrate to 0.6 mmol / L and the enzyme concentration on the activity of the enzyme, luteolin And its complexes on xanthine oxidase inhibition types are reversible inhibition by fixing xanthine oxidase concentration of 10μg / mL, with Lineweaver-Burk double reciprocal mapping, luteolin and its complexes xanthine oxidase The type of inhibition appears to be competitive inhibition.