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为探讨 6号染色体长臂上与非小细胞肺癌发生相关的微卫星位点 ,应用多重PCR对 41例非小细胞肺癌中 6号染色体长臂上的 36个微卫星位点进行扩增。PCR产物应用聚丙烯酰胺凝胶电泳分离 ,电泳结果用GeneScanTM、GenotyperTM软件进行分析。结果发现各个位点有明显不同的LOH频率 ,除D6S1 5 79在 41例非小细胞肺癌中未发现杂合性缺失外 ,其余 35个位点均有至少一个位点发生突变 ,LOH频率从 3.5 7%到 75 %不等 ,总LOH频率为 78% ( 32 /41 ) ,其中D6S30 2位点的LOH频率最高 ( 75 % )。LOH频率超过 2 0 %的位点共有 1 4个 ,主要分布在 2个区域。其中有 6个位点 :D6S45 8( 2 1 .43% )、D6S1 694( 2 6.92 % )、D6S1 71 7( 35 .71 % )、D6S1 5 65 ( 4 0 % )、D6S30 2 ( 75 % )、D6S1 70 6( 36.36% )分布在 6q2 1附近 ,具体区域是 6q1 6.3 q2 1 ;有 5个位点 :D6S1 5 5 0 ( 38.46% )、D6S2 64( 2 0 % )、D6S1 5 85 ( 2 5 % )、D6S446( 33.33% )、D6S2 81( 30 .77% )分布在 6q2 7附近 ,具体区域是 6q2 6～q2 7。因此 6q2 1、q2 7附近可能存在与非小细胞肺癌发生相关的已知或未知肿瘤抑制基因
To investigate the microsatellite loci associated with non-small cell lung cancer on the long arm of chromosome 6, multiplex PCR was used to amplify 36 microsatellite loci on the long arm of chromosome 6 in 41 non-small cell lung cancer. PCR products were separated by polyacrylamide gel electrophoresis, electrophoresis results were analyzed using GeneScanTM, GenotyperTM software. The results showed that there was a significantly different LOH frequency in all loci except for D6S1 5 79 heterozygous deletions in 41 cases of non-small cell lung cancer, the other 35 loci have at least one site mutation, LOH frequency from 3.5 The total LOH frequency was 78% (32/41), among which the frequency of LOH at D6S30 2 site was the highest (75%). A total of 14 LOH sites with frequencies above 20% are mainly distributed in 2 areas. There are 6 sites: D6S45 8 (21.43%), D6S1 694 (26.92%), D6S1 71 7 (35.71%), D6S1 5 65 (40%), D6S30 2 , D6S1706 (36.36%) distributed around 6q2 1, the specific region is 6q1 6.3 q2 1; There are 5 sites: D6S1505 (38.46%), D6S264 (20%), D6S1585 5%), D6S446 (33.33%), D6S2 81 (30.77%) distributed around 6q2 7, the specific region is 6q2 6 ~ q2 7. Therefore, 6q21, q2 7 near the existence of non-small cell lung cancer may be related to the known or unknown tumor suppressor gene