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用 0～ 70 0mmol/LNaCl和 0～ 1 50mmol/LNa2 CO3对羊草 (Aneu rolepidiumchinense (Trin)Kitag .)苗进行胁迫处理 ,测定其N及几种有机渗透调节物的积累情况 .结果表明 :羊草对两种盐胁迫的反应明显不同 .对于NaCl,可耐受的最大强度为 60 0mmol/L ;对于Na2 CO3,可耐受的最大强度为 1 2 5mmol/L .相同Na+胁强下 ,Na2 CO3胁迫使羊草苗体内含N量下降的幅度明显大于NaCl胁迫 .NaCl胁迫下 ,脯氨酸及可溶性糖含量随Na+胁强增大呈直线式平缓上升 ,在Na2 CO3胁迫下则呈明显的曲线变化 ;在胁强较低时 ,脯氨酸可溶性糖含量无明显变化 ,但当胁强超过 87 5mmol/L时 ,二者均转而急剧上升 ,其中尤以脯氨酸含量的变化最明显 .NaCl胁迫时 ,羊草中有机酸及柠檬酸含量无明显变化 ,而Na2 CO3胁迫则使二者含量明显上升 ,并证明积累的有机酸以柠檬酸为主 .从实验结果看来 ,中性盐 (NaCl)与碱性盐 (Na2 CO3)对羊草的胁迫机制以及羊草对其生理适应机制存在明显差异 .
The seedlings of Aneu rolepidium chinense (Trin) Kitag seedlings were treated with 0 ~ 70 0mmol / L NaCl and 0 ~ 150mmol / L Na2CO3, and the accumulation of N and several organic osmotic adjusting agents were measured.The results showed that: The response of grass to salt stress was obviously different.The maximum tolerable strength was 60 0mmol / L for NaCl and the maximum tolerable intensity for Na2CO3 was 115mmol / L. Under the same Na + stress, Na2 Under CO3 stress, the decrease of N content in L. chinense seedlings was significantly greater than that of NaCl stress.NaCl stress increased the content of proline and soluble sugar with the increase of Na +, and then increased linearly with Na2CO3 stress The curve of soluble sugar content showed no significant change at lower stress level, but when the stress intensity exceeded 87 5mmol / L, both of them turned sharply, especially the change of proline content was the most obvious .NaCl stress, Leymus chinensis organic acids and citric acid content did not change significantly, while the Na2 CO3 stress make both significantly increased, and the accumulation of organic acids to citric acid.From the experimental results, the neutral Stress mechanism of Leymus chinensis with NaCl and Na2CO3 Chinensis and its significantly different physiological adaptation mechanisms.