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以B37-C和B37-N的玉米原生质体和叶片为试材,研究HMC毒素对两种同核异质玉米B37细胞凋亡的诱导作用。经Hoechst33258荧光染色表明:HMC毒素处理后的玉米原生质体均出现细胞凋亡现象,在荧光显微镜下可观察到凋亡小体、染色质边集或环状染色质等凋亡特征。原生质体的细胞凋亡率,B37的C细胞质显著高于N细胞质玉米,且凋亡率随处理浓度和处理时间的增加而上升。用HMC毒素处理玉米叶片,明显出现DNA-ladder现象时所需的毒素浓度C、N两种细胞质均为200μg/mL;但C细胞质所需的时间为6 h,而N细胞质则需9 h。用荧光显微观察与凝胶电泳分析所获得的结果一致,均表现出C细胞质对毒素较为敏感,先出现细胞凋亡;N细胞质对毒素的敏感性低于C细胞质,出现细胞凋亡的时间较晚。
The protoplasts and leaves of B37-C and B37-N were used as materials to study the induction of apoptosis by HMC to two isoforms of corn B37 cells. Fluorescence staining with Hoechst 33258 showed that apoptosis occurred in protoplasts of HMC toxins after treatment with HMC. The apoptotic bodies, chromatin margins and circular chromatin were observed under fluorescent microscope. Protoplast apoptosis rate, B37 C cytoplasm was significantly higher than N cytoplasmic maize, and the apoptosis rate increased with the treatment concentration and treatment time. Toxicity of the toxin to C-N and N-cytoplasm was 200μg / mL for the treatment of corn leaves with HMC toxin. However, the time required for C-cytoplasm was 6 h and that of N-cytoplasm was 9 h. Fluorescence microscopy and gel electrophoresis analysis of the results obtained are consistent, showing that the cytoplasm of C is more sensitive to toxins, first apoptosis; N cytoplasm toxin sensitivity lower than the C cytoplasm, apoptosis occurred time Later.