采用Ｂｅｒｔｈｏｌｄ法制备肿瘤坏死因子（ＴＮＦα）壳聚糖微球，并测定微球的一些基本特征参数。结果表明，微球粒径主要分布在 １． ２～３． ５ μｍ内，载药量为 ４５００ Ｕ／ｍｇ，包封率为 ９５． ４％。体外释放实验表明，壳聚糖微球具有显著的缓释作用，是生物大分子药物很好的载体。ＴＮＦα壳聚糖微球能显著减轻小鼠瘤重，延长小鼠存活期，降低药物的毒副作用，与空白组及ＴＮＦα组比较，均有极显著意义（均为产Ｐ＜０．０１）。 Tumor necrosis factor (TNFα) chitosan microspheres were prepared by Berthold method and some basic characteristic parameters of microspheres were determined. The results showed that the size of microspheres mainly distributed in 1. 2 ~ 3. 5 μm, drug loading 4500 U / mg, encapsulation efficiency of 95. 4%. In vitro release experiments showed that chitosan microspheres have a significant sustained-release effect, is a good carrier of biological macromolecular drugs. TNFα chitosan microspheres can significantly reduce the tumor weight, prolong the survival of mice and reduce the side effects of drugs, compared with the blank group and TNFα group, both of which have very significant (all P <0.01).