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目的考察接枝不同官能团(-COOH、-OH、两性基团)的聚对苯二甲酸丁二醇酯(PBT)膜对血小板黏附及血小板功能的影响,选择适用于过滤去除浓缩血小板中白细胞的材料。方法利用紫外改性的方法,将不同单体包括丙烯酸(AA)、丙烯酸羟乙酯(HEA)和N-(3-磺丙基)-N-甲基丙烯酸基氧乙基-N,N-二甲基铵内铵盐(SMDB)接枝到PBT表面,得到接枝不同官能团的PBT膜,包括PBT-AA(-COOH)、PBT-HEA(-OH)、PBT-SMDB(两性基团);通过傅里叶红外光谱、水接触角及表面粗糙度等指标对改性PBT的理化性能做表征,采用扫描电镜观察改性PBT对血小板的黏附,并通过检测PBT改性前后的血小板聚集率、低渗休克相对变化、CD62p表达率等指标,考察改性PBT对血小板功能的影响。结果在红外光谱的1 047和1 043 cm-1处出现了C-O-C的弯曲振动峰和O=S=O的对称伸缩振动峰,提示HEA和SMDB接枝到了PBT膜表面。扫描电镜显示:未改性的PBT膜表面黏附的血小板数明显多于改性PBT膜表面,且可以观察到明显的伪足。血小板黏附数在接枝不同官能团的PBT膜表面顺序为:-COOH>两性基团>-OH。未改性PBT组(n=6)与对照组(未接触材料的血小板)(n=6)比较:血小板最大聚集率(%)为87.22±2.10 vs 82.36±2.11,低渗休克相对变化率(%)为79.33±1.27 vs 77.12±1.09,CD62p表达率增加(%)为9.78±0.58 vs 17.45±1.25(P<0.05)。在接枝不同官能团的改性PBT膜中,PBT-HEA(n=6)对血小板聚集和血小板活化等功能影响最小[血小板最大聚集率(93.34±1.45)%、血小板低渗休克相对变化率(81.26±1.48)%、CD62p表达率(12.51±0.85)%]。结论表面接枝HEA的聚酯对血小板的黏附及功能影响明显小于接枝其他官能团的材料,或可作为用于浓缩血小板过滤去除白细胞的新材料。
Objective To investigate the effect of polybutylene terephthalate (PBT) grafted with different functional groups (-COOH, -OH, amphoteric groups) on platelet adhesion and platelet function, and to select suitable for the filtration and removal of leukocytes in concentrated platelets material. Methods Different monomers including acrylic acid (AA), hydroxyethyl acrylate (HEA) and N- (3-sulfopropyl) -N-methylacryloxyethyl-N, N- The grafting of SMDB onto the surface of PBT resulted in PBT membranes grafted with different functional groups, including PBT-AA (-COOH), PBT-HEA (-OH), PBT-SMDB The physical and chemical properties of modified PBT were characterized by Fourier transform infrared spectroscopy, water contact angle and surface roughness. The adhesion of modified PBT to platelets was observed by scanning electron microscopy. The platelet aggregation rate , The relative changes of hypotonic shock, CD62p expression rate and other indicators to investigate the effect of modified PBT on platelet function. Results The flexural vibrational peak of C-O-C and the symmetrical stretching vibrational peak of O = S = O appeared at 1 047 and 1 043 cm-1 in IR spectra, suggesting that HEA and SMDB were grafted on the surface of PBT membrane. Scanning electron microscopy showed that the number of platelets adhered to the unmodified PBT membrane surface was significantly more than that of the modified PBT membrane, and obvious pseudopodia were observed. Platelet adhesion number in the grafting of different functional groups PBT membrane surface sequence: -COOH> amphoteric group> -OH. The maximum platelet aggregation rate (%) was 87.22 ± 2.10 vs 82.36 ± 2.11 in untreated PBT group (n = 6) and the control group (untreated platelet) (n = 6) %) Was 79.33 ± 1.27 vs 77.12 ± 1.09, the expression of CD62p increased (%) was 9.78 ± 0.58 vs 17.45 ± 1.25 (P <0.05). PBT-HEA (n = 6) had the least effect on the function of platelet aggregation and platelet activation in the modified PBT membranes grafted with different functional groups [maximum platelet aggregation rate (93.34 ± 1.45)%, relative change rate of platelet hypotonic shock 81.26 ± 1.48)%, CD62p expression (12.51 ± 0.85)%]. Conclusions The surface-grafted polyesters of HEA have significantly less effect on platelet adhesion and function than those grafted with other functional groups, or can be used as new materials for concentrating PLT to remove leukocytes.