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目的:研究中药扶正解毒颗粒(FJG)对硫酸镍(NiSO4)染毒大鼠肾组织核因子-κB(NF-κB)活化的影响。方法:50只Wistar大鼠采用NiSO4 2.5 mg.kg-1 ip(连续7 d,此后间日1次)制备肾损伤模型,随机分为NiSO4组(模型组)、FJG高、中、低剂量组(分别为20,10,5 g.kg-1.d-1),二硫基丁二酸(DMSA),50 mg.kg-1.d-1)组。另设NS组(空白对照)及FJG对照组(FJG 10 g.kg-1.d-1)。各组大鼠ig 1次/d,共4周。测定各组大鼠血尿素氮(BUN)、肌酐(SCr)及24 h尿蛋白定量(24 h-UP),免疫组化SP法观察肾组织NF-κB活化。结果:NiSO4组大鼠出现肾功能损伤,NF-κB活化:肾小球(34.62±9.11)%,肾小管(62.45±14.78)%;FJG大剂量组NF-κB活化:肾小球(2.08±0.64)%,肾小管(11.48±3.39)%,明显低于NiSO4组(P<0.01),肾功能损伤亦明显减轻(P<0.05,P<0.01)。结论:FJG可能通过抑制肾组织NF-κB活性的异常升高而发挥拮抗镍性肾损伤的作用。
Objective: To investigate the effect of Fuzheng Jiedu Granule (FJG) on activation of nuclear factor-κB (NF-κB) in kidney of rats exposed to nickel sulfate (NiSO4). Methods: Fifty Wistar rats were randomly divided into NiSO4 group (model group), FJG high dose group, middle dose group, and low dose group with NiSO4 2.5 mg.kg-1 ip (for 7 consecutive days, (20, 10, 5 g.kg-1.d-1, respectively), dithiosuccinic acid (DMSA), 50 mg.kg-1.d-1). Another NS group (blank control) and FJG control group (FJG 10 g.kg-1.d-1). Each rat ig 1 / d, a total of 4 weeks. Blood urea nitrogen (BUN), creatinine (SCr) and 24 h urinary protein (UA) were measured in all groups. The activation of NF-κB in renal tissue was observed by immunohistochemistry SP method. Results: The renal function injury, NF-κB activation in the NiSO4 group were as follows: glomeruli (34.62 ± 9.11)%, tubules (62.45 ± 14.78)%; NF-κB activation in glomeruli of FJG high dose group 0.64%, and tubule (11.48 ± 3.39)%, respectively, which were significantly lower than those in NiSO4 group (P <0.01). Renal dysfunction was also relieved (P <0.05, P <0.01). CONCLUSION: FJG may play an antagonistic role against nickel kidney injury by inhibiting the abnormal increase of NF-κB activity in renal tissues.