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用高效液相色谱法测定配合物的表观稳定常数,Horvath等曾有报道。只要待测物与其配合物的保留时间有差异即可用本法测定。如样品中含有杂质,可通过色谱柱分离,不需事先纯化,这对于腺嘌呤核苷三磷酸(ATP)和腺嘌呤核苷二磷酸(ADP)这些易降解的生化物质表观稳定常数的测定尤为有利。 实验部分 (一) 仪器与试剂 1.仪器 岛津LC-4A色谱仪;色谱柱:Nucleosil-ODS,4×250mm,7μm(大连化学物理研究所仪器厂装)。 2.试剂 ATP,上海生化试制商店;ADP,上海生
The apparent stability constants of the complexes were determined by HPLC, Horvath et al. As long as the analyte and its complex retention time difference can be determined by this method. If the sample contains impurities and can be separated by column without prior purification, this is an indication of the apparent stability constants of these biodegradable materials, adenine nucleotide triphosphates (ATP) and adenosine diphosphate (ADP) Particularly beneficial. Experimental part (a) instruments and reagents 1 instrument Shimadzu LC-4A chromatograph; column: Nucleosil-ODS, 4 × 250mm, 7μm (Dalian Institute of Chemical Physics Instrument Factory). 2. Reagents ATP, Shanghai Biochemical trial store; ADP, Shanghai Health