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目的观察不同剂量的阿法骨化醇对8月龄未交配♀SD大鼠骨组织形态计量学的影响,并探讨阿法骨化醇对大鼠皮质骨和松质骨影响的差异性。方法阿法骨化醇分别按0.005、0.025、0.05、0.1μg·kg-1·d-14种不同剂量,连续给大鼠灌胃120d,同时设棉籽油空白对照组和基础组。120d后处死大鼠,取右侧胫骨上端和中段不脱钙骨组织包埋、磨片、染色,进行骨组织形态计量学测量和计量。结果阿法骨化醇0.1μg·kg-1·d-1治疗组与棉籽油空白对照组比较,胫骨上段骺端松质骨骨小梁面积百分数(%B.Ar)增加,骨小梁的宽度(Tb.Wi)升高,骨小梁的分离度(Tb.Sp)下降;增加骨芽的形成;松质骨量呈剂量依赖性增加;皮质骨骨外膜面荧光周长百分数(%Ps-L.Pm)明显增加,骨表面骨形成率(Ps-BFR/BS)上升;骨内膜面荧光周长百分数(%En-L.Pm)明显下降和骨内膜面吸收周长(%En-Er.Pm)下降,但皮质骨骨量的增加不明显。结论对正常8月龄未交配♀SD大鼠给予0.1μg·kg-1·d-1阿法骨化醇灌胃120d,具有促骨合成作用,表现在刺激皮质骨骨外膜的骨形成和促进松质骨骨芽形成,大鼠松质骨骨量增加。而抗骨吸收作用表现在抑制骨小梁骨表面和皮质骨骨内膜面的骨吸收,且抑制骨吸收作用大于骨形成,产生正性的内膜骨平衡状态。阿法骨化醇对松质骨的效应明显强于皮质骨。
Objective To observe the effect of different doses of alfacalciferol on bone histomorphometry in 8-month-old unpaired SD rats and to explore the difference of the effects of alfacalcidol on cortical bone and cancellous bone in rats. Methods Alfacalciferol were administered to rats at different dosages of 0.005, 0.025, 0.05 and 0.1 μg · kg-1 · d-1 for 14 days, respectively. Meanwhile, cottonseed oil blank control group and basic group were established. After 120 days, the rats were sacrificed, and the upper part of the right tibia and the middle part of non-decalcified bone were embedded, polished and stained. The bone histomorphometry was measured and measured. Results Compared with cottonseed oil control group, the percentage of trabecular bone area (% B.Ar) in cancellous bone at the distal epiphysis of the tibia was increased in the group treated with alfacalcidyl 0.1μg · kg -1 · d -1 (Tb.Wi) increased, the trabecular bone separation (Tb.Sp) decreased; increased the formation of bone buds; the amount of cancellous bone increased in a dose-dependent manner; the percentage of% (Ps-BFR / BS) increased; the percentages of peri-luminal fluorescence perimeter (% En-L.Pm) decreased significantly and the periosteal surface resorption perimeter % En-Er.Pm) decreased, but the increase of cortical bone mass was not obvious. CONCLUSIONS: Orally administered 0.1 μg · kg-1 · d-1 alfacalcidol for 8 days in normal 8-month-old SD rats can induce bone formation and promote osteogenesis in the cortical bone Promote cancellous bone bud formation, increase the amount of cancellous bone in rats. The anti-bone resorption is shown to inhibit the bone trabecular bone surface and the cortical bone endometrial absorption, and inhibition of bone resorption than bone formation, resulting in a positive endometrial bone balance. Alfacaloids on cancellous bone was significantly stronger than cortical bone.