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目的 :探讨胎脾LAK细胞对不同的T淋巴白血病细胞的杀伤作用。方法 :采用低浓度的重组人白介素 2(rhIL 2 )诱导的胎脾LAK细胞杀伤两种不同的T淋巴白血病细胞系MOLT 4和HPB ALL的51Cr释放实验。结果 :发现不同的LAK效应细胞和靶细胞比 (E∶T)条件下 ,胎脾LAK细胞对两种不同的T淋巴白血病细胞杀伤能力随E∶T的增大而增强 (P <0 .0 1)。相同E∶T比时 ,对MOLT 4的杀伤显著强于对HPB ALL的杀伤 (P <0 .0 0 1)。各E∶T比的平均杀伤率MOLT 4为 (5 8.2 4± 12 .93) % ,HPB ALL为 (34.33± 13 .2 0 ) % (P <0 .0 0 1)。结论 :胎脾LAK细胞对不同的T淋巴白血病细胞的杀伤能力存在着差异 ,其可能与T淋巴白血病细胞的分化程度相关。E∶T比越大 ,杀伤能力越强
Objective: To investigate the killing effect of fetal spleen LAK cells on different T lymphoblastic leukemia cells. Methods: The 51Cr release assay of two different T lymphoblastic leukemia cell lines, MOLT 4 and HPB ALL, was performed using fetal lung LAK cells induced with low concentrations of recombinant human interleukin 2 (rhIL 2). Results: It was found that the killing ability of fetal LAK cells on two different T lymphoid leukemia cells increased with the increase of E: T (P <0. 0) at different LAK effector to target cell ratio (E: T) 1). At the same E: T ratio, the killing of MOLT 4 was significantly stronger than that of HPB ALL (P <0.01). The average killing rate of each E: T ratio was (5 8.2 4 ± 12 .93)% for MOLT 4 and (34.33 ± 13.2%) for HPB ALL (P <0.01). Conclusion: There are differences in the killing ability of fetal LAK cells on different T lymphoblastic leukemia cells, which may be related to the differentiation of T lymphoid leukemia cells. E: T ratio is greater, the stronger the ability to kill