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目的观察不同剂量反式二羟环氧苯并(a)芘(anti-BPDE)处理对人支气管上皮细胞(16HBE)POLK基因表达的影响。方法分别以0.25、0.50、1.00、2.00μmol/L的反式-BPDE1次或3次处理16HBE细胞,采用TaqmanMGB探针实时定量聚合酶链反应方法相对定量POLK和POLZ基因表达,同时对反式-BPDE转化的16HBE及肺癌H1299细胞POLK基因表达进行了分析。结果反式-BPDE1次处理及3次处理可诱导正常16HBEPOLK基因高表达。反式-BPDE转化16HBE和肺癌H1299细胞POLK基因表达明显增高,分别是正常16HBE的2.25和5.49倍。结论反式-BPDE处理可致16HBEPOLK基因高表达。
Objective To observe the effect of different doses of anti-BPDE on the expression of POLK in human bronchial epithelial cells (16HBE). Methods 16HBE cells were treated with 1, 2 or 3 trans-BPDE at 0.25, 0.50, 1.00, 2.00 μmol / L, respectively. The expression of POLK and POLZ genes was quantified by TaqmanMGB probe real time quantitative polymerase chain reaction BPDE transformed 16HBE and lung cancer H1299 cell POLK gene expression was analyzed. Results Trans-BPDE1 treatment and 3 treatments induced high expression of normal 16HBEPOLK gene. Trans-BPDE 16HBE and lung cancer H1299 cell POLK gene expression was significantly higher, respectively, 2.25 and 5.49 times the normal 16HBE. Conclusion Trans-BPDE treatment can cause high expression of 16HBEPOLK gene.