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目的探讨衰老大鼠阴茎组织转化生长因子β(TGF-β)基因表达与补肾通络疏肝方在阴茎勃起中的关系。方法将32只雄性大鼠随机分为衰老组、补肾通络疏肝方组、丙酸睾酮组与对照组(2月龄大鼠),其中补肾通络疏肝方组和丙酸睾酮组分别给予补肾通络疏肝方及丙酸睾酮治疗,于24 d取阴茎组织,用PCR检测其TGF-βmRNA的表达。结果衰老组TGF-βmRNA的表达较其他3组增强,而表现TGF-βmRNA减弱的组,由少到多依次排定为对照组、丙酸睾酮组及补肾通络疏肝方组;对照组、丙酸睾酮组及补肾通络疏肝方组其TGF-βmRNA内控量较少,与衰老组比较有显著性差异(P<0.01),丙酸睾酮组及补肾通络疏肝方组之间无显著性差异(P>0.05)。结论补肾通络疏肝方可能通过调节TGF-βmRNA表达而在阴茎勃起中发挥重要作用。
Objective To investigate the relationship between the gene expression of transforming growth factor β (TGF-β) in the penis of aging rats and the effect of Bushen Tonglu Shugan Recipe on penile erection. Methods Thirty-two male rats were randomly divided into three groups: aging group, Bushen Tongluo Shugan Recipe group, testosterone propionate group and control group (2-month-old rats). Among them, Bushen Tonglu Shugan Decoction group and testosterone propionate group respectively After treatment with Bushen Tongluo Shugan Recipe and testosterone propionate, the penis tissue was taken at 24 days and the expression of TGF-βmRNA was detected by PCR. Results The expression of TGF-β mRNA in aging group was higher than that in other three groups, while the group that showed decreased TGF-β mRNA was ranked as control group, testosterone propionate group and Bushen Tongluo Shugan Recipe group from control group to control group. In the testosterone propionate group and the Bushen Tongluo Shugan Recipe group, the amount of TGF-β mRNA was less controlled, and there was a significant difference from the aging group (P<0.01). There was no difference between the testosterone propionate group and the Bushen Tongluo Shuganfang group. Significant difference (P>0.05). Conclusion Bushen Tongluo Shugan Decoction may play an important role in penile erection by regulating TGF-βmRNA expression.