论文部分内容阅读
从种稻红壤中分离微生物菌株,经紫外诱变后得到了3株高效的稻秆纤维素降解菌株,分别为细菌YB20、真菌F9和YF15.经鉴定,菌株YB20为枯草芽孢杆菌(Bacillus subtilis sp.),菌株F9和YF15均为绿色木霉(Trichoderma viride sp.).对3株纤维素降解菌株产酶特性进行了初步研究,确定了菌株的最佳产酶条件,菌株YB20、F9和YF15的胞外羧甲基纤维素(CMC)酶活性高达2.67、2.78和3.56(mg/mL).30 min.产酶稳定性试验表明,菌株YB20、F9、YF15的CMC酶活性均具有较好的稳定性.纤维素降解菌剂培养试验结果表明,3株菌混合培养对秸秆腐解具有较好的协同效应,稻秆腐解率可达到35.02%,比对照提高了4.43倍.大田应用试验显示,与不施用菌剂的对照相比,施用纤维素降解菌剂在一定程度上提高了晚稻产量.
Microbial strains were isolated from the paddy red soils and three highly efficient strains of rice straw cellulose degradation were obtained, which were bacteria YB20, F9 and YF15, respectively.The strain YB20 was identified as Bacillus subtilis sp ), F9 and YF15 were all Trichoderma viride sp. The optimum conditions of enzyme production were determined for three cellulase-degrading strains, and the strains YB20, F9 and YF15 The CMCase activities of strains YB20, F9 and YF15 were both 2.67, 2.78 and 3.56 (mg / mL) .30 min. The results showed that the three strains mixed culture had a good synergistic effect on the decomposition of straw, the decomposing rate of rice straw reached 35.02%, which was 4.43 times higher than that of the control.The field application test showed , Compared with the control without the use of fungi, the application of cellulose-degrading fungi increased the yield of late rice to a certain extent.