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目的:探讨肿瘤坏死因子启动子(TNF- 30 8)基因多态性与哮喘发病之间的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR- RFL P)方法研究了5 0例哮喘患者和80例健康对照者TNF- 30 8等位基因和基因型的分布情况。结果:TNF- 30 8等位基因在哮喘组和健康对照组之间的分布差异无显著性(χ2 =0 .0 4 7,P>0 .0 5 )。同时,TNF- 30 8的3种基因型(GG、GA和AA)在哮喘组(45 /5 0 ,5 /5 0和0 /5 0 )和健康对照组(71/80、9/80和0 /80 )的分布差异也无显著性(χ2 =0 .0 4 9,P>0 .0 5 )。结论:TNF- 30 8基因多态性与哮喘发病无关联。
Objective: To investigate the relationship between the polymorphism of tumor necrosis factor promoter (TNF-30 8) gene and the pathogenesis of asthma. Methods: The distribution of TNF-30 8 alleles and genotypes in 50 asthma patients and 80 healthy controls were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: There was no significant difference in the distribution of TNF-30 8 between the asthma group and the healthy controls (χ2 = 0.047, P> 0.05). At the same time, the three genotypes of TNF-30 8 (GG, GA and AA) were significantly higher in the asthma group (45/50, 5/50 and 0/500) and in the healthy controls (71 / 80,9 / 80 and 0/80) was also not significantly different (χ2 = 0.409, P> 0.05). Conclusion: TNF-30 8 gene polymorphism is not associated with asthma.