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目的:探讨萝卜硫素(SFN)对肾小管上皮细胞(HK-2)的氧化应激及核转录因子E2相关因子2(Nrf2)/血红素加氧酶(HO)-1信号通路的影响。方法:体外培养HK-2细胞,将细胞分为空白对照组、H_2O_2处理组、H_2O_2+10μmol·L~(-1)SFN组、H_2O_2+20μmol·L~(-1)SFN组和H_2O_2+40μmol·L~(-1)SFN组;测定HK-2细胞中丙二醛(MDA)、一氧化氮(NO)、谷胱甘肽(GSH)的含量及超氧化物歧化酶(SOD)的活性;RT-PCR和Western Blot法分别检测Nrf2和HO-1 mRNA及蛋白表达水平。结果:与空白对照组相比,H_2O_2处理组中MDA、NO含量明显升高,GSH水平和SOD酶活性显著降低(P<0.05);经高、中、低3种浓度的萝卜硫素处理后MDA、NO含量显著降低,GSH水平显著升高,SOD酶活性也显著升高,Nrf2和HO-1mRNA及蛋白表达明显上调(P<0.05)。结论:萝卜硫素有抗HK-2细胞氧化应激损伤的作用,其作用机制可能与激活Nrf2/HO-1信号通路有关。
Objective: To investigate the effects of radish sulphate (SFN) on the oxidative stress and the expression of Nrf2 / HO-1 in renal tubular epithelial cells (HK-2). Methods: HK-2 cells were cultured in vitro and divided into blank control group, H 2 O 2 treated group, H 2 O 2 + 10μmol·L -1 SFN group, H 2 O 2 + 20μmol·L -1 SFN group and H 2 O 2 + 40μmol · L -1 SFN group. The content of malondialdehyde (MDA), nitric oxide (NO) and glutathione (GSH) and the activity of superoxide dismutase (SOD) in HK-2 cells The mRNA and protein levels of Nrf2 and HO-1 were detected by RT-PCR and Western Blot respectively. Results: Compared with the blank control group, the contents of MDA and NO in the H 2 O 2 treatment group were significantly increased, while the levels of GSH and SOD activity were significantly decreased (P <0.05). After being treated with high, medium and low concentrations of radish sulfur MDA and NO were significantly decreased, GSH level was significantly increased, SOD activity was significantly increased, Nrf2 and HO-1 mRNA and protein expression was significantly increased (P <0.05). Conclusion: Sulfasalazine has anti-oxidative stress-induced injury in HK-2 cells, which may be related to the activation of Nrf2 / HO-1 signaling pathway.