聚合酶链反应 (PCR)是在引物介导及酶促作用下 ,在体外连续扩增特定基因的分子生物学技术。 PCR技术是由美国 Cefus公司人类遗传学部的 Sairi于 1985年研究成功的 ,并在以后短短几年内迅速发展 ,被广泛用于医学基础研究和临床诊断。本文就该技术的原理、特点及其在病毒性视网膜 Polymerase chain reaction (PCR) is a molecular biology technique for continuous amplification of specific genes in vitro under primer-mediated and enzymatic action. PCR technology was successfully researched by Sairi of Cefus Human Genetics Division in the United States in 1985 and developed rapidly in a few short years. It is widely used in medical basic research and clinical diagnosis. This article on the principle of the technology, its characteristics and its role in the viral retina