目的:测定清肺止咳颗粒中木兰脂素、秦皮甲素、秦皮乙素的含量。方法:采用HPLC法,使用十八烷基硅烷键合硅胶色谱柱DiamonsiL C18(250 mm×4.6 mm,5μm)。测定木兰脂素的流动相为乙腈-水(40∶60),流速1 mL.min-1,检测波长为278 nm;测定秦皮甲素、秦皮乙素的流动相为乙腈-0.1%磷酸溶液(8∶92),流速1.0 mL.min-1,检测波长为334 nm。结果:木兰脂素、秦皮甲素、秦皮乙素进样量分别在0.22～1.76μg(r=0.9998)、0.102～0.306μg(r=0.9997)、0.23～0.69μg(r=0.9997)范围内线性关系良好;平均回收率为99.9%、97.7%、100.0%,RSD为1.4%、2.0%、1.8%。结论:所建立的分析方法可作为清肺止咳颗粒的质控标准。 Objective: To determine the contents of magnolol, aesculin and aesculetin in Qingfei Zhike Granule. Methods: The DiamonsiL C18 column (250 mm × 4.6 mm, 5 μm) was prepared by HPLC using octadecylsilane bonded silica gel column. The mobile phase of magnolynin was acetonitrile-water (40:60), the flow rate was 1 mL.min-1, the detection wavelength was 278 nm. The mobile phase of aesculin and aesculetin was acetonitrile-0.1% phosphoric acid solution 8:92). The flow rate was 1.0 mL.min-1 and the detection wavelength was 334 nm. Results: The injection volumes of magnolin, aesculin and aesculetin were linear in the range of 0.22-1.76μg (r = 0.9998), 0.102-0.306μg (r = 0.9997), 0.23-0.69μg (r = 0.9997) The average recoveries were 99.9%, 97.7% and 100.0% with RSDs of 1.4%, 2.0% and 1.8%, respectively. Conclusion: The established analytical method can be used as a quality control standard Qingfei Zhike granules.