论文部分内容阅读
目的探讨利用链脲佐菌素(STZ)建立理想糖尿病性勃起功能障碍大鼠模型。方法30只SD大鼠,随机分为5组,分别为对照组、注射STZ40mg/kg组、60mg/kg组、80mg/kg组、100mg/kg组,每组6只,分别记录4d、1周、2周、3周的空腹血糖、阿朴吗啡诱导阴茎的勃起次数及体质量。结果4个时间点间的空腹血糖有显著性差异(P=0.001),不同STZ注射组别间的空腹血糖、勃起次数及体质量改变存在显著性差异(P<0.001,P=0.045及P<0.001),阿朴吗啡阴茎勃起实验的勃起次数及体质量的改变在4个时间点间不存在显著性差异(P=0.306和P=0.628)。结论最佳成糖尿病模型的STZ注射剂量应为60mg/kg,筛选糖尿病性勃起功能障碍模型的阿朴吗啡勃起实验筛选时间应定在注射STZ后的2周左右。
Objective To explore the use of streptozotocin (STZ) to establish an ideal diabetic erectile dysfunction rat model. Methods Thirty SD rats were randomly divided into five groups: control group, STZ40mg / kg group, 60mg / kg group, 80mg / kg group and 100mg / kg group, with 6 rats in each group. , Fasting blood glucose of 2 weeks and 3 weeks, the number of penis erection and body weight induced by apomorphine. Results There were significant differences in fasting blood glucose between the four time points (P = 0.001). There were significant differences in fasting blood glucose, erectile frequency and body weight between different STZ injection groups (P <0.001, P = 0.045 and P < 0.001). There was no significant difference (P = 0.306 and P = 0.628) between the erection frequency and weight change of apomorphine penile erection test at 4 time points. Conclusion STZ injection should be 60mg / kg in the best diabetic model. The screening time of apomorphine erection screening for diabetic erectile dysfunction model should be about 2 weeks after injecting STZ.