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目的:建立HPLC双波长切换法对二妙丸中盐酸小檗碱、苍术素的含量测定,该方法有利于二妙丸的含量测定及质量控制。方法:采用反相高效液相色谱法;以0.02mol/L磷酸二氢钾(用磷酸调PH3.0)-乙腈(色谱纯)流动相梯度洗脱;流速:1.0m L/min;柱温:30℃;检测波长:270nm(0~10min,盐酸小檗碱)、340nm(10~20min,苍术素);结果:HPLC双波长切换法可同时测定二妙丸中盐酸小檗碱、苍术素的含量,盐酸小檗碱和苍术素分别在14.63~292.5μg/m L、0.54~10.75μg/m L和峰面积呈良好的线性关系,平均加样回收率(N=6)分别为99.8196%、99.2808%。RSD分别为0.6163%、2.1763%。结论:该方法准确、可靠、专属性强,为二妙丸的质量控制提供参考。
OBJECTIVE: To establish a HPLC dual wavelength switching method for the determination of berberine hydrochloride and atractylodes in Ermiao Pill. The method is beneficial to the determination and quality control of Ermiao Pill. Methods: The mobile phase was eluted with 0.02mol / L KH2PO4 (adjusted to pH3.0 with phosphoric acid) -acetonitrile (chromatographic purity). The flow rate was 1.0m L / min. The column temperature : 30 ℃; detection wavelength: 270nm (0 ~ 10min, berberine hydrochloride), 340nm (10 ~ 20min, atractylodes macrocephala); Results: HPLC dual wavelength switching method can simultaneously determine berberine hydrochloride, , The content of berberine and atractylodes were 14.63 ~ 292.5μg / m L, 0.54 ~ 10.75μg / m L and the peak area showed a good linear relationship, the average recovery rate (N = 6) were 99.8196% , 99.2808%. RSD were 0.6163%, 2.1763% respectively. Conclusion: The method is accurate, reliable and specific. It provides a reference for the quality control of Ermiao Wan.