目的通过检测大鼠高原适应后各组织中EPAS1基因表达量的变化,为高原获得性习服提供分子机理研究基础及为急慢性高原病的发病机制进一步研究提供资料。方法通过实时PCR法检测平原组大鼠和高原适应(4350 m,7 d)组大鼠心、肝、脑、肺、肾组织中EPAS1基因的表达量。结果与平原组相比,大鼠在高原适应过程中各组织的EPAS1表达量均有升高,其中心、脑中表达量变化最大,分别为6.03倍和10.71倍。结论在高原适应性方面,各组织器官对适应性的贡献并不一致,为高原实验性研究提供依据。 Objective To investigate the changes of EPAS1 gene expression in different tissues after plateau adaptation in rats and to provide molecular basis for the study of acquired suits in plateau and provide information for further study on the pathogenesis of acute and chronic altitude sickness. Methods Real-time PCR was used to detect the expression of EPAS1 gene in heart, liver, brain, lung and kidney of rats in plateau group and plateau-adapted group (4350 m, 7 d) Results Compared with the placenta group, the expression of EPAS1 was increased in all tissues during the plateau adaptation, and the expression of EPAS1 was the highest in the center and in the brain, which were 6.03 and 10.71 times, respectively. Conclusion In terms of plateau adaptability, the contribution of tissues and organs to the adaptability is not consistent, providing the basis for the experimental study of plateau.