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目的:探讨促血管生成素2(Ang-2)在不同程度急性肺损伤(ALI)中的作用。方法:清洁级SD大鼠共40只,随机分为4组:正常对照组,不同剂量大肠杆菌脂多糖(LPS)组(3个剂量组:2 mg/kg、4 mg/kg及8 mg/kg),每组平均10只。HE染色光镜观察肺组织标本病理改变并进行肺损伤评分,Western blot检测各组大鼠血浆中Ang-2的表达情况。结果:LPS可致肺泡间隔增宽、出血及大量炎性细胞浸润等急性肺损伤病理改变,LPS各组的肺损伤评分明显高于正常对照组(P<0.01);LPS不同剂量与肺损伤评分呈量效依赖关系(P<0.05)。LPS不同剂量组的血浆中Ang-2蛋白表达较空白对照组增高(P<0.01),LPS不同剂量与Ang-2蛋白表达水平呈量效依赖关系(P<0.05)。血浆中Ang-2的蛋白表达与肺组织炎症程度积分呈正相关(r=0.862,P<0.05)。结论:Ang-2参与大鼠内毒素性急性肺损伤的病理过程,且血浆Ang-2水平与急性肺损伤程度呈正相关。
Objective: To investigate the role of angiopoietin 2 (Ang-2) in acute lung injury (ALI). Methods: Forty clean SD rats were randomly divided into four groups: normal control group, different doses of E. coli lipopolysaccharide (LPS) group (3 doses: 2 mg / kg, 4 mg / kg and 8 mg / kg), with an average of 10 in each group. The pathological changes of lung tissue were observed with HE staining and the lung injury scores were scored. The expression of Ang-2 in plasma was detected by Western blot. Results: LPS could induce the pathological changes of acute lung injury such as widening of alveolar septum, hemorrhage and a large number of inflammatory cell infiltration. The lung injury score of LPS group was significantly higher than that of normal control group (P <0.01) Showed a dose-dependent relationship (P <0.05). The plasma levels of Ang-2 protein in LPS group were higher than those in blank control group (P <0.01). The dose-dependent relationship between LPS and Ang-2 protein level was significant (P <0.05). Plasma Ang-2 protein expression was positively correlated with lung inflammation score (r = 0.862, P <0.05). Conclusion: Ang-2 is involved in the pathogenesis of endotoxic acute lung injury in rats, and the plasma Ang-2 level is positively correlated with the degree of acute lung injury.