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目的:探讨α-干扰素对体外培养的骨巨细胞瘤细胞碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)及受体Bek表达与生物学行为的影响,为骨巨细胞瘤术后复发转移的防治探索新途径。方法:采用不同浓度的α-干扰素诱导体外培养的第三代骨巨细胞瘤细胞,观察α-干扰素对瘤细胞生物学行为的影响,流式细胞术与细胞-酶联免疫吸附法分析α-干扰素诱导48h后瘤细胞凋亡情况及Bek/bFGF表达的变化。结果:浓度为1000IU/ml以上的α-干扰素作用10～12h左右,瘤细胞即出现退缩现象,生长明显受到抑制;至48h,瘤细胞数目明显减少,且出现大量细胞碎片。α-干扰素可诱导第三代骨巨细胞瘤细胞发生凋亡,其凋亡率随α-干扰素作用浓度增加而递增,呈明显的剂量依赖关系。α-干扰素可抑制第三代骨巨细胞瘤细胞bFGF和Bek的表达,其抑制强度也随α-干扰素作用浓度的增加而增加,呈明显的量效关系。当α-干扰素作用浓度达到1000IU/ml以上时,其诱导凋亡与抑制Bek/bFGF表达的效应均发生显著性变化。结论:α-干扰素对体外培养的第三代骨巨细胞瘤细胞具有明确的抗肿瘤效应,可作为骨巨细胞瘤术后辅助治疗,以防治和减少其复发转移。
Objective: To investigate the effect of α-interferon on the expression of basic fibroblast growth factor (bFGF) and its receptor Bek and the biological behavior of giant cell tumor of bone in vitro. Prevention and control to explore new ways. Methods: The third generation of giant cell tumor cells were induced by different concentrations of α-interferon. The effects of α-interferon on the biological behavior of tumor cells were observed. Flow cytometry and cell-enzyme-linked immunosorbent assay Apoptosis of tumor cells and the expression of Bek / bFGF after induced by. Results: The effect of α-interferon above 1000IU / ml for 10 ~ 12h showed that the tumor cells retreated and the growth was obviously inhibited. At 48h, the number of tumor cells decreased significantly, and a large number of cell debris appeared. Interferon-alpha induced the apoptosis of the third generation of giant cell tumor of bone cells, and its apoptosis rate increased with the increase of α-interferon concentration in a dose-dependent manner. Interferon-α inhibited the expression of bFGF and Bek in the third generation of giant cell tumor of bone, and its inhibitory intensity also increased with the concentration of α-interferon, showing a dose-effect relationship. When α-interferon concentration reached above 1000 IU / ml, the effect of inducing apoptosis and inhibiting Bek / bFGF expression both had significant changes. CONCLUSION: Alpha interferon has a clear anti-tumor effect on the third generation of giant cell tumor of bone cells cultured in vitro. It can be used as an adjuvant therapy for the treatment of giant cell tumor of bone to prevent and reduce its recurrence and metastasis.