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目的在体外利用7名志愿者的新鲜粪便作为来源,选择菊粉作为对照,研究干酪乳杆菌LC2W与鼠李糖乳杆菌Y37胞外多糖对人体肠道菌群的影响。方法利用PCR-DGGE(变性梯度凝胶电泳)方法分析样品中菌群组成,高效液相色谱分析培养前后胞外多糖组分变化。结果与菊粉相比,粪便菌群在添加乳杆菌胞外多糖培养后,Parabacteroides类群细菌明显增加,4名志愿者粪便样品中出现明显的双歧杆菌条带,两种乳杆菌胞外多糖对粪便菌群的影响差异无统计学意义;而添加菊粉培养后,Bacteroides类群细菌的条带明显增加。两种乳杆菌胞外多糖中主要是大分子量的部分被利用。结论干酪乳杆菌LC2W与鼠李糖乳杆菌Y37的胞外多糖在体外有调节粪便菌群的作用。
OBJECTIVE To study the effects of Lactobacillus casei LC2W and Lactobacillus rhamnosus Y37 exopolysaccharide on human intestinal microflora in vitro using fresh faeces of seven volunteers as source. Methods The bacterial community composition was analyzed by PCR-DGGE (denaturing gradient gel electrophoresis) and the changes of exopolysaccharides composition were analyzed by high performance liquid chromatography. Results Compared with inulin, the bacteria in Parabacteroides group increased significantly in excreted feces after addition of Lactobacillus exopolysaccharides. There were obvious bands of Bifidobacterium in four fecal samples and two kinds of Lactobacillus extracellular polysaccharides The influence of fecal flora was not statistically significant; however, the bacterial bands of Bacteroides significantly increased after addition of inulin. Two Lactobacilli exopolysaccharides are mainly used in large molecular weight fractions. Conclusion Lactobacillus casei LC2W and Lactobacillus rhamnosus Y37 exopolysaccharide can regulate fecal flora in vitro.