目的建立HPLC测定龙柴方及其组方药材甘草中5种指标成分含量的方法,控制该方药的质量。方法 ODS C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈和0.05%的磷酸水溶液,梯度洗脱;柱温为30℃;流速1 mL.min-1;检测波长237 nm。结果此方法可以使样品达到基线分离,阴性样品无干扰,甘草苷、异甘草苷、甘草素、甘草酸和异甘草素分别在0.156 8～1.568μg(r=0.999 7)、0.157 1～1.571μg(r=0.999 8)、0.155 8～1.558μg(r=0.999 6)、0.187 1～1.871μg(r=0.996 9)和0.158 1～1.581μg(r=0.999 5)内与各自峰面积积分值呈良好的线性关系;平均回收率分别为101.47%(RSD=1.272%)、101.09%(RSD=1.937%)、101.14%(RSD=2.388%)、100.38%(RSD=1.448%)及100.86%(RSD=1.759%)(n=5)。结论该方法分离良好、精密准确,可用于龙柴方及其组方药材甘草的质量控制。 OBJECTIVE To establish a HPLC method for the determination of five index components in Long Chai Fang and its compound prescription licorice, and to control the quality of the prescription. Methods ODS C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and 0.05% phosphoric acid. The gradient elution was carried out. The column temperature was 30 ℃. The flow rate was 1 mL.min-1. The detection wavelength was 237 nm. Results The method was able to separate the samples from baseline and the negative samples were free from interference. The concentrations of glycyrrhizin, isoliquiritigenin, glycyrrhizin, glycyrrhizin and isoliquiritigenin were 0.156 8 ~ 1.568 μg (r = 0.999 7) and 0.157 1 ~ 1.571 μg (r = 0.999 8), 0.155 8 ~ 1.558μg (r = 0.999 6), 0.187 1 ~ 1.871μg (r = 0.996 9) and 0.158 1 ~ 1.581μg (r = 0.999 5) The average recoveries were 101.47% (RSD = 1.272%), 101.09% (RSD = 1.937%), 101.14% (RSD = 2.388%), 100.38% (RSD = 1.448%) and 100.86% = 1.759%) (n = 5). Conclusion The method is well separated, accurate and accurate, and can be used for the quality control of Long Chai Fang and its group prescription licorice.