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目的:研究1-磷酸鞘氨醇(S1P)对淋巴管内皮细胞(HDLEC)的生物学特性的调节作用及其调节机制。方法:应用RT-PCR方法检测HDLEC是否表达SPK及S1P受体,应用MTT法、细胞扩散盒技术检测S1P对HDLEC增殖、迁移特性的影响,并用Western印迹方法检测S1P对HDLEC的MAPK信号通路的调节。结果:HDLEC表达SPK及S1P受体,包括S1P1,S1P2,S1P3和S1P4。外源性S1P对HDLEC没有促增殖作用,但可促进其迁移,并可通过S1P1和S1P2诱导MAPK快速磷酸化。结论:初步研究结果提示,S1P可以影响淋巴管内皮细胞的生物学特性,有可能成为调控淋巴管生成及其功能的新靶点。
AIM: To investigate the regulatory effect of sphingosine-1-phosphate (S1P) on the biological characteristics of lymphatic endothelial cells (HDLEC) and its regulatory mechanism. Methods: RT-PCR was used to detect whether HDLEC expresses SPK and S1P receptors. The effects of S1P on the proliferation and migration of HDLEC were detected by MTT assay and cell diffusion kit. The regulation of MAPK signal pathway of HDLEC by S1P was detected by Western blotting . Results: HDLEC expresses SPK and S1P receptors, including S1P1, S1P2, S1P3 and S1P4. Exogenous S1P did not promote proliferation of HDLEC but promoted its migration and induced rapid phosphorylation of MAPK through S1P1 and S1P2. Conclusion: The preliminary results suggest that S1P can affect the biological characteristics of lymphatic endothelial cells and may be a new target of regulating lymphangiogenesis and its function.