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目的探讨Survivin、Caspase-3表达对鼻咽癌发生、发展及放疗后复发的影响,并研究两者间的相互关系。方法采用免疫组化SP法检测31例放疗后复发鼻咽癌、30例鼻咽非角化型鳞癌、30例鼻咽粘膜慢性炎标本中Survivin及Caspase-3的表达情况。结果 Survivin、Caspase-3表达的阳性率,鼻咽粘膜慢性炎分别为23.33%和96.67%,在鼻咽非角化型鳞癌中分别为73.33%和16.67%,在放疗后复发鼻咽癌分别为83.87%、22.58%,两者在三组中的表达,差异有统计学意义(P<0.001),在鼻咽非角化型鳞癌组与放疗后复发组相比差异无统计学意义(P>0.05)。在放疗后复发鼻咽癌中,Survivin与Caspase-3的表达有显著负相关性(r=-0.398,P<0.05)。结论 Survivin的激活以及Caspase-3的失活参与了鼻咽癌的发生发展过程,Survivin在胞核的阳性表达可能与放射抗性相关。
Objective To investigate the effect of Survivin and Caspase-3 expression on the occurrence and development of nasopharyngeal carcinoma (NPC) and the relapse after radiotherapy, and to study the relationship between them. Methods Immunohistochemical SP method was used to detect the expression of Survivin and Caspase-3 in 31 cases of nasopharyngeal carcinoma after radiotherapy, 30 cases of nasopharyngeal non-keratinizing squamous cell carcinoma and 30 cases of nasopharyngeal mucosal chronic inflammation. Results The positive rates of Survivin and Caspase-3 expression were 23.33% and 96.67% in nasopharyngeal mucosa and 73.33% and 16.67% in nasopharyngeal non-keratinizing squamous cell carcinoma respectively. The recurrence of nasopharyngeal carcinoma after radiotherapy (83.87%, 22.58%). The difference between the two groups was statistically significant (P <0.001). There was no significant difference between the nasopharyngeal non-keratinizing squamous cell carcinoma group and the recurrent radiotherapy group ( P> 0.05). There was a significant negative correlation between the expression of Survivin and Caspase-3 in nasopharyngeal carcinoma after radiotherapy (r = -0.398, P <0.05). Conclusion The activation of Survivin and the inactivation of Caspase-3 are involved in the occurrence and development of NPC. The positive expression of Survivin in the nucleus may be related to radioresistance.