乳酸杆菌为应用最早、研究最多的益生菌种之一,能够调节肠道微生物区系的平衡,增强机体的免疫力和抵抗力。S-层蛋白在乳酸杆菌黏附过程中起重要作用,因此乳酸杆菌中的S-层蛋白成为研究热点。本文对乳酸杆菌S-层蛋白的结构、分离提纯方法、基因克隆情况和益生功能做了详细阐述。 Lactobacillus, one of the earliest and most studied probiotic strains, regulates the balance of gut microbiota and enhances the body’s immunity and immunity. The S-layer protein plays an important role in the adhesion of Lactobacillus, so the S-layer protein in Lactobacillus has become a research hotspot. In this paper, the structure of Lactobacillus S-layer protein, separation and purification methods, gene cloning and probiotic function were elaborated.