用免疫印迹技术已证明,获得性大疱性表皮松解症(EBA)循坏自身抗体是直接抗基底膜的一种独特的蛋白。该技术可用于EBA的诊断研究。EBA抗体的靶抗原是Ⅶ型胶原的羧基端区。这种胶原是新近发现的,是形成锚纤维的主要成分,体外培养的真皮成纤维细胞和角朊细胞均能合成这种 It has been shown by immunoblotting that acquired autoantibodies to epidermolysis bullosa (EBA) are a unique protein that directly binds to the basement membrane. This technique can be used to diagnose EBA. The target antigen of the EBA antibody is the carboxy terminal region of type VII collagen. This collagen is newly discovered and is the main component forming anchor fibers. Dermal fibroblasts and keratinocytes cultured in vitro can synthesize this