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目的通过检测肠道病毒71型(EV71)重症感染死亡病例脑、肺组织中Toll样受体(TLR)7、8的表达,探讨TLR7、8的表达在EV71感染发病中的意义。方法以9例因EV71感染死亡的儿童病例为研究对象(EV71组),7例因意外或非感染性疾病死亡的儿童病例作为对照。采集两组死亡病例尸检时的脑、肺组织,应用免疫组化法检测两组肺和脑组织中TLR7、8的阳性表达;采用积分光密度值(IOD)对TLR7、8的表达进行半定量分析。结果免疫组化结果示EV71组TLR7、8在肺和脑组织中的表达呈强阳性,其IOD值明显高于对照组(P<0.05)。TLR7和TLR8在EV71组的肺、脑组织中的表达差异无统计学意义(P>0.05)。结论 TLR7、8在EV71重症感染死亡病例的脑、肺组织中呈高表达状态,提示TLR7、8可能参与了EV71重症感染患者脑及肺部损害的发病机制。
Objective To detect the expression of Toll-like receptor (TLR) 7 and 8 in brain and lung tissues of deaths from severe infection of enterovirus 71 (EV71) and to explore the significance of the expression of TLR7 and 8 in the pathogenesis of EV71 infection. Methods Nine children died of EV71 infection (EV71 group) and seven children died of accidental or non-infectious disease as control. The brain and lung tissues were collected from autopsy of two deaths. Immunohistochemistry was used to detect the expression of TLR7, 8 in both lung and brain tissues. The integral optical density (IOD) analysis. Results The results of immunohistochemistry showed that the expression of TLR7, 8 in EV71 group was strongly positive in lung and brain tissues, and the IOD value of EV71 group was significantly higher than that of the control group (P <0.05). The expression of TLR7 and TLR8 in the lung and brain of EV71 group showed no significant difference (P> 0.05). Conclusion TLR7 and 8 are highly expressed in brain and lung tissues of EV71 patients with severe infection, suggesting that TLR7 and 8 might be involved in the pathogenesis of brain and lung damage in patients with EV71 severe infection.