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目的对抗运动疲劳功能食品动力源胶囊进行安全性评价研究。方法采用限量法测定急性毒性试验,给予KM小鼠受试物最大剂量1 0 g/kg·BW后,观察14天内有无动物死亡,计算半数致死量(LD50)。应用30 d喂养试验观察可能的亚慢性毒性,分别设2670 mg/kg·BW、1330 mg/kg·BW、670 mg/kg·BW)剂量组和蒸馏水空白对照组,灌胃给予SD大鼠受试物30 d,观察和记录SD大鼠的生长发育情况、摄食量、食物利用率、血液学指标、血液生化指标和脏器病理检查。结果急性毒性试验观察期内受试动物未出现任何异常表现及死亡,表明样品无毒;在30 d喂养试验中各组大鼠给予不同剂量的动力源胶囊30 d后,其血液生化学检查、脏器称量结果表明,各检验项目的实验组与对照组比较均差异不显著。病理组织学检查结果表明对照组和各剂量组雄、雌性大鼠的肝、脾、肾、胃、肠、睾丸(卵巢)均未见与实验因素有关的病理组织学变化,表明30 d喂养试验未见其对受试动物有毒性损害作用。结论动力源胶囊使用安全。
Objective To evaluate the safety of functional fatigue-power capsule for motor exercise. Methods The acute toxicity test was conducted by the limit test. After the maximum dose of KM was given to the test substance at 10 g / kg · BW, no animals were killed within 14 days and the LD50 was calculated. The 30-day feeding test was used to observe the possible sub-chronic toxicity. The rats were divided into two groups: 2670 mg / kg BW, 1330 mg / kg BW and 670 mg / kg BW, and distilled water blank control group. After 30 days, the growth and development, food intake, food utilization rate, hematological indexes, blood biochemical indexes and pathological examination of SD rats were observed and recorded. Results Acute toxicity test During the observation period, there were no abnormalities and deaths in the test animals, indicating that the samples were non-toxic. After 30 d of feeding experiment, rats in each group were given different doses of motility capsule for 30 days. The blood biochemical tests, Organ weighing results show that the test items of the experimental group and the control group were no significant difference. Histopathological examination showed that there was no histopathological changes in the liver, spleen, kidney, stomach, intestine and testis (ovary) of the male and female rats in the control group and each dose group, indicating that the 30-day feeding test Did not see its toxic effects on animals tested. Conclusion Power capsule safe to use.