【摘 要】
：
To identify EST-SSR molecular markers, 41,986 cattle UniGene sequences from NCBI were mined for analyzing SSRs. A total of 1,831 SSRs were identified from 1,666
【机 构】
：
College of Animal Science and Technology,National Center for Molecular Genetics and Breeding of Anim
【基金项目】
：
国家高技术研究发展计划(863计划);Eleventh Five-year Plan on Dairy Industry Key Problem;Animal and Plant Breeding Project of Agriculture and Forestry;Program of Innovation Fund of IAS
论文部分内容阅读
To identify EST-SSR molecular markers, 41,986 cattle UniGene sequences from NCBI were mined for analyzing SSRs. A total of 1,831 SSRs were identified from 1,666 ESTs, which represented an average density of 19.88 kb per SSR. The frequency of EST-SSRs was 4.0%. The dinucleotide repeat motif was the most abundant SSR, accounting for 54%, followed by 22%, 13%, 7% and 4%, respec-tively, for tri-, hexa-, penta- and tetra-nucleotide repeats. Depending upon the length of the repeat unit, the length of microsatellites varied from 14 to 86 bp. Among the di- and tri-nucleotide repeats, AC/TG (57%) and AGC (12%) were the most abundant type. Annotation of EST-SSRs was also carried out. Three hundred primer pairs were randomly designed using Prime Premier 5.0 program and Oligo 5.0 for further experimental validation.
其他文献
Rhodococcus erythropolis USTB-03 is a promising bacterial strain for the biodesulfurization of dibenzothiophene (DBT) via a sulfurspecific pathway in which DBT
A large array of soil properties influences plant growth response to phosphorus (P) fertilizer input in acid soils.We carried out a pot experiment using three c
Organic carbon (OC), total nitrogen (TN), and 210Pb in core sediment were measured to quantify the burial of organic carbon and the relative importance of alloc
The title compound, [Cu3(dmpzm)4(μ-1,3-dca)2(dca)4]·2H2O (dmpzm = 1,1-methyle- nebis(3,5-dimethyl-1H-pyrazole), dca = dicyanamide) 1·2H2O, was synthesized vi
The paper describes a controllable layer-by-layer (LBL) self-assembly modification technique of multi-walled carbon nanotubes(MWNTs) and poly(diallyldimethylamm
A new method was developed for the determination of cefradine by extraction-flotation of CuSCN. The experiment indicatedthat in the presence of 0.20 mol/L NaOH
背景3年前，澳大利亚政府开始鼓励在基层医疗卫生机构中采用远程视频医疗服务，即利用远程医疗设备，专家通过患者的全科医师、护士、土著卫生人员、养老机构为患者提供咨询。乡村
Ambient volatile organic compounds (VOCs) were sampled at six sites in Beijing in the summer of 2004 and analyzed byGCMS. The chemical reactivities of 73 quanti
Concentrations of copper (Cu), lead (Pb), chromium (Cr), mercury (Hg), and arsenic (As) were measured in arid agricultural and irrigated agricultural soils coll
作为全科医生，要想给患者提供有效的临床保健建议，就需要有详尽的、多学科的临床知识。面对妇女健康领域的诸多问题，我们需要学习并掌握全面的、最新的妇女保健知识，澳大利亚 Mon