论文部分内容阅读
以披碱草×野大麦杂种F_1幼穗为外植体,诱导出胚性愈伤组织,建立了植株再生体系,对影响愈伤组织诱导、分化、生根的基本培养基、激素种类及质量浓度等进行筛选。结果表明:愈伤组织诱导以MS为基本培养基+3mg/L 2,4-D较好;外植体在MS+3mg/L 2,4-D+30g/L蔗糖+7g/L琼脂的诱导培养基上经过20d培养,小穗愈伤组织诱导率达28.3%;愈伤组织的分化培养基为MS+1mg/L 6-BA+1mg/L NAA+30g/L蔗糖+7g/L琼脂,愈伤组织分化率达78%;生根培养基以1/2MS+0.5mg/L IBA+0.5mg/L NAA+蔗糖+7g/L琼脂为最佳,生根率可达86.7%,移栽后成活率可达100%。
The embryogenic callus was induced from the young F1 hybrids of Elymus dahuricus × Hordeum brevisubulatum, and the plant regeneration system was established. The basic medium, the type of hormone and the concentration of hormone, which affected the callus induction, differentiation and rooting, Screening. The results showed that callus induction with MS as the basic medium was better than that with 3mg / L 2,4-D. The explants were cultured in MS + 3mg / L 2,4-D + 30g / L sucrose + 7g / L agar The induction rate of spikelet callus reached 28.3% after 20d culture on induction medium. The differentiation medium of callus was MS + 1mg / L 6-BA + 1mg / L NAA + 30g / L sucrose + 7g / L agar , The rate of callus differentiation reached 78%; rooting medium with 1/2 MS + 0.5mg / L IBA + 0.5mg / L NAA + sucrose + 7g / L agar was the best, the rooting rate reached 86.7% Rate up to 100%.