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以耐光抑制粳亚种品种０２４２８，０２９和对光抑制敏感的籼亚种品种３０３７，Ｐａｌｇｈａｒ及其正反交Ｆ１杂种为材料，研究了水稻籼粳亚种抗光破坏能力的差异与Ｄ１蛋白和叶黄素循环的关系．用链霉素（ ＳＭ）处理证明，当 Ｄ１蛋白合成受到抑制时， ＰＳⅡ光化学效率（ Ｆｖ／Ｆｍ）降低，而叶黄素循环受到促进、非光化学猝灭（ｑＮ）增加；用二硫苏糖醇（ＤＴＴ）处理证明，当叶黄素循环受到抑制时， ｑＮ下降并导致 Ｄ１蛋白更多的降解和 Ｆｖ／Ｆｍ的大幅度下降．与籼亚种相比，粳亚种的 Ｄ１蛋白维持能力较强，Ｆｖ／Ｆｍ降低的幅度较小，抗光破坏能力较强；杂种Ｆ１的上述指标在双亲值之间且偏向其母本．实验结果支持质基因编码的Ｄ１蛋白周转能力是水稻耐光抑制的重要生理基础的观点，并为选配耐光抑制杂种稻的亲本和开拓高光效育种途径提供生理依据．
In order to study the relationship between light-induced damage abilities of rice indica-japonica and D1 protein and its hybrids, the light-tolerant japonica subspecies 02428,029 and indica subspecies 3037, Palghar and their F1 hybrids were studied. Lutein cycle relationship. Treatment with streptomycin (SM) demonstrated that the photochemical efficiency of PSII (Fv / Fm) was reduced and the xanthophyll cycle was promoted and the non-photochemical quenching (qN) was increased when the synthesis of D1 was inhibited. With dithiothreitol The alcohol (DTT) treatment demonstrated that when the lutein cycle was inhibited, qN decreased and led to more degradation of the D1 protein and a substantial decrease in Fv / Fm. Compared with indica subspecies, japonica subspecies D1 had stronger ability to maintain D1, Fv / Fm decreased less and its ability to resist light damage was stronger than those from indica subspecies. The experimental results support the viewpoint that the turnover ability of the D1 protein encoded by the pMD gene is an important physiological basis of photosynthetic light suppression in rice and provide the physiological basis for the selection of the parents of the hybrid rice with photostabilistic inhibition and the development of high light efficiency breeding approaches.