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目的 :观察排钱草总生物碱对免疫性肝纤维化大鼠肝脏胶原沉积和转化生长因子 β1(TGF β1)表达的影响 ,研究其对肝纤维化形成的抑制作用 ,并探讨可能的作用机制。方法 :设立模型对照组、药物对照组、排钱草总生物碱高、中、低剂量组和正常对照组 ,以腹腔注射猪血清方法诱导大鼠肝纤维化动物模型。排钱草总生物碱高、中、低剂量组大鼠在注射猪血清的同时分别灌服排钱草总生物碱 3 0mg/kg ,2 0mg/kg ,10mg/kg ,1次 /d ,共 8周。大鼠肝脏HE染色 ,观察各组大鼠肝组织的炎性坏死与胶原纤维沉积变化 ;免疫组化观察大鼠肝纤维化形成过程中腓钱草总生物碱对肝脏表达Ⅰ、Ⅲ、Ⅳ型胶原蛋白及TGF β1的影响。结果 :与正常对照组比较 ,模型组大鼠肝脏出现典型的肝纤维化表现 ,肝脏胶原纤维间隔广泛形成 ,肝小叶与肝窦内胶原增生沉积明显 ,Ⅰ ,Ⅲ ,Ⅳ型胶原 (1. 2 8± 2 .5 9vs13 .82± 6. 5 5 ,1 .0 0± 1. 2 2vs 14. 69± 7 .16,1 0 3± 1 46vs 12. 44± 6/ 89,P值均 <0 . 0 0 1)及TGF β1表达明显增多。高、中、低剂量排钱草总生物碱均可以明显减轻大鼠肝脏内胶原纤维增生沉积 (P <0 . 0 5 ) ,抑制肝脏Ⅰ ,Ⅲ ,Ⅳ型胶原蛋白 (P值均 <0 0 5 )及TGF β1的合成表达。结论 :排钱草总生物碱通过抑制肝纤维化大鼠肝
OBJECTIVE: To observe the effects of total alkaloids from Detox gypsophila on hepatic collagen deposition and transforming growth factor-β1 (TGF-β1) expression in hepatic fibrosis rats, to study its inhibitory effect on hepatic fibrosis formation, and to explore possible mechanisms of action. . METHODS: A model control group, a drug control group, a total alkaloid alkaloid high-, medium-, low-dose group, and a normal control group were established. An animal model of hepatic fibrosis was induced by intraperitoneal injection of pig serum. The rats in the high, medium, and low dose groups of D. platyphylla total alkaloids were fed with D. chinensis total alkaloids 30 mg/kg, 20 mg/kg, 10 mg/kg, and 1/d respectively for the same time as the pig serum was injected. 8 weeks. Rat hepatic HE staining was used to observe the changes of inflammatory necrosis and collagen fiber deposition in liver tissue of rats in each group. Immunohistochemistry was used to observe the expression of type I, III, IV in the liver of total alkaloids of S. chinensis in liver fibrosis. Effects of collagen and TGF β1. RESULTS: Compared with the normal control group, livers of the model group exhibited typical hepatic fibrosis, extensive formation of hepatic collagen fibril septa, and hyperplasia of collagen in hepatic lobule and hepatic sinusoids. Collagen type I, III, and IV (1.2) 8±2.5 9vs13.82±6.55,1.0 0±1.2 2vs 14. 69± 7 .16,1 0 3± 1 46vs 12. 44± 6/ 89, all P values < 0 0 01) and TGF β1 expression increased significantly. High alkaloids, medium and low doses of D. chinensis total alkaloids all significantly reduced the deposition of collagen fibrosis in rat liver (P < 0.05) and inhibited collagen I, III, and IV of liver (P < 0 0 5) and synthetic expression of TGFβ1. Conclusion : The total alkaloids of Dinodendrum hepatica inhibit hepatic fibrosis in rat liver