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目的考察地西泮及复方地西泮(地西泮+薄荷醇)鼻腔给药对纤毛的毒性作用。方法以盐酸麻黄碱作阴性对照药,去氧胆酸钠作阳性对照药,考察地西泮、复方地西泮鼻腔给药对蟾蜍上颚纤毛持续运动时间(Persistent Vibration Duration,PVD)、纤毛持续运动百分率(Percentage of Persistent Vibration,PPV)、纤毛输送速率和蟾蜍上颚黏膜形态的影响。结果显微镜下观察,地西泮组、复方地西泮组对蟾蜍上颚纤毛形态无明显影响;地西泮组、复方地西泮组给药后PVD分别为(597.4±70.2)min、(606.2±55.4)min,与生理盐水组比较均无显著性差异(P>0.05);除1%盐酸麻黄碱组外,其余各组纤毛输送速率与生理盐水相比,差异有显著性(P<0.05)。结论地西泮、复方地西泮经鼻腔给药后,对蟾蜍鼻黏膜纤毛的毒性轻微,为鼻腔给药提供参考。
Objective To investigate the toxic effects of diazepam and nasal diazepam (diazepam + menthol) on the cilia. Methods Ephedrine hydrochloride as a negative control drug, sodium deoxycholate as a positive control drug, investigated diazepam, nasal compound dipyridamole toad pawpaw sustained movement time (Persistent Vibration Duration, PVD), continuous ciliary movement Percentage of Persistent Vibration (PPV), ciliary delivery rate, and toad palate mucosal morphology. Results Microscope showed that the diazepam group and the compound diazepam group had no significant effect on the shape of the toad palate. The PVD after diazepam group and the compound diazepam group were (597.4 ± 70.2) min, (606.2 ± 55.4) min, there was no significant difference compared with saline group (P> 0.05). Except for 1% ephedrine hydrochloride group, there was a significant difference in the rate of cilia delivery compared with saline (P <0.05) . Conclusion Diazepam and compound diazepam after nasal administration have slight toxicity to nasal mucosa of toad and provide a reference for nasal administration.