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目的建立三七叶抗抑郁活性提取物PnGL中皂苷类成分含量测定的方法。方法采用香荚醛-高氯酸比色法,分别以人参二醇及人参皂苷Rb3为对照品,测定总皂苷的含量。采用HPLC法,Ultimate AQ-C18色谱柱,甲醇-水(69∶31)为流动相,检测波长203 nm,柱温为25℃,同时测定PnGL中主要成分人参皂苷Rb1、Rb2、Rc、Rb3的含量。结果以人参二醇及人参皂苷Rb3为对照品所测定的总皂苷含量,分别为97.25%、95.76%,差异不大。HPLC检测基线平稳,人参皂苷Rb1、Rb2、Rc、Rb3分离良好,分离度1.5以上,平均加样回收率分别为99.24%(RSD=1.63%)、96.41%(RSD=1.21%)、96.81%(RSD=0.86%)、100.16%(RSD=1.84%)。结论含量测定方法准确、重复性好,可为PnGL质量控制提供依据。
Objective To establish a method for determination of the content of saponins in PnGL from the anti-depressant activity of Panax notoginseng. Methods Determination of total saponin content with the determination of pivalic aldehyde - perchloric acid colorimetric method, respectively, ginseng diol and ginsenoside Rb3 as a reference substance. The mobile phase consisted of Ultimate AQ-C18 column, methanol-water (69:31), detection wavelength of 203 nm and column temperature of 25 ℃. The main constituents of PnGL were identified as Rb1, Rb2, Rc and Rb3 content. Results The contents of total saponins determined by ginseng diol and ginsenoside Rb3 were 97.25% and 95.76% respectively, with no significant difference. The baseline of HPLC was stable. The average separation recoveries of Ginsenosides Rb1, Rb2, Rc and Rb3 were 99.24% (RSD = 1.63%), 96.41% (RSD = 1.21%) and 96.81% RSD = 0.86%), 100.16% (RSD = 1.84%). Conclusion The content determination method is accurate and reproducible, which can provide the basis for PnGL quality control.