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目的:观察夏龙方对胃癌细胞SGC-7901黏附、侵袭、移动等转移生物学行为的影响。方法:体外培养SGC-7901细胞,分为夏龙方50,100,200 mg·L-1(低、中、高剂量)组和对照组,采用Cytoselect 48-Well Cell Adhesion Assay检测胃癌细胞与基质黏附能力;利用Transwell侵袭转移模型评价夏龙方对胃癌细胞侵袭能力的影响、划痕实验观察夏龙方对SGC-7901细胞移动能力的影响。结果:终浓度100,200 mg·L-1夏龙方能显著降低SGC-7901细胞黏附能力,50～200 mg·L-1可抑制SGC-7901细胞侵袭,终浓度50 mg·L-1夏龙方可显著抑制SGC-7901细胞迁移。结论:夏龙方可降低SGC-7901细胞的黏附能力,侵袭能力,抑制细胞迁移。
OBJECTIVE: To observe the effect of Xia Long Fang on the biological behaviors of gastric cancer SGC-7901 such as adhesion, invasion and migration. Methods: SGC-7901 cells were cultured in vitro and divided into three groups: XiaLongFang 50,100,200 mg · L-1 (low, middle and high dose) and control group. Cytoselect 48-Well Cell Adhesion Assay Transwell invasion and metastasis model was used to evaluate the effect of Xiaolong Fang on invasion ability of gastric cancer cells. Scratch experiment was carried out to observe the effect of Xia Long Fang on the migration ability of SGC-7901 cells. Results: Xialongfang at a concentration of 100,200 mg · L-1 could significantly reduce the cell adhesion of SGC-7901 cells, and 50 ~ 200 mg · L-1 could inhibit the invasion of SGC-7901 cells at a final concentration of 50 mg · L-1 Can significantly inhibit SGC-7901 cell migration. Conclusion: Xiaolong Fang can reduce SGC-7901 cell adhesion, invasion and inhibition of cell migration.