论文部分内容阅读
目的 :通过检测胃癌及癌前病变组织端粒酶的活性 ,探讨在这些病变演化中端粒酶活性表达的规律及其意义。方法 :采用端粒酶TRAP ELISA法定量检测 33例胃癌、8例胃粘膜重度异型增生、5例轻度异型增生、7例胃粘膜肠上皮化生、19例慢性浅表性胃炎组织端粒酶活性。结果 :由慢性浅表性胃炎→胃粘膜肠上皮化生→轻度异型增生→重度异型增生→胃癌 ,端粒酶阳性率逐渐增高 ,分别为 0 %、42 9%、40 0 %、75 0 %、84 0 %。端粒酶阳性率在胃癌及重度异型增生组明显高于其它各组 (P <0 .0 0 5 )。慢性浅表性胃炎与肠上皮化生及轻度异型增生组比较也有显著差异 (P <0 .0 2 5 ) ,而胃癌与重度异型增生组比较差异无显著性。结论 :端粒酶在胃癌的发生中可能具有重要意义。追踪端粒酶阳性的胃癌前病变患者有利于早期发现胃癌。
OBJECTIVE: To investigate the expression of telomerase activity and its significance in the evolution of these lesions by detecting telomerase activity in gastric cancer and precancerous lesions. Methods: Telomerase TRAP ELISA was used to detect 33 cases of gastric cancer, 8 cases of severe gastric dysplasia, 5 cases of mild dysplasia, 7 cases of gastric mucosal intestinal metaplasia, 19 cases of chronic superficial gastritis tissue telomerase active. Results: The positive rate of telomerase was gradually increased from chronic superficial gastritis to gastric mucosal intestinal metaplasia to mild dysplasia to severe dysplasia to gastric cancer, which were respectively 0%, 42 9%, 40 0% and 75 0 %, 84 0%. The positive rate of telomerase in gastric cancer and severe dysplasia group was significantly higher than other groups (P <0.05). There was also significant difference between chronic superficial gastritis and intestinal metaplasia and mild dysplasia (P <0.05), but there was no significant difference between gastric cancer and severe dysplasia. Conclusion: Telomerase may play an important role in the carcinogenesis of gastric cancer. Patients with gastric precancerous lesions that track telomerase positive are good at early detection of gastric cancer.