论文部分内容阅读
目的分析白介素-8(IL-8)在痛风性关节炎(GA)患者外周血中的表达变化,探讨其临床意义。方法选择急性痛风性关节炎(AGA组)、非急性痛风性关节炎间歇期(NAGA组)和健康体检者(HC组)各43例。利用RTPCR和Western blot方法分别检测外周血单个核细胞(PBMCs)中IL-8mRNA和蛋白表达,再用ELISA法检测各组血浆IL-8水平。结果 IL-8mRNA在AGA组PBMCs中的表达高于NAGA组和HC组(均P<0.01),在NAGA组的表达高于HC组(P<0.01)。PBMCs中的IL-8蛋白水平在AGA组高于NAGA组和HC组(均P<0.01),且HC组最低,但NAGA组和HC组比较差异无统计学意义(P>0.05)。血浆中IL-8水平在AGA组和NAGA组显著高于HC组(均P<0.01),NAGA组显著高于HC组(P<0.05)。结论 IL-8在GA患者的外周血中异常表达,提示IL-8在GA的病理机制中可能发挥重要的作用。
Objective To analyze the expression of interleukin-8 (IL-8) in peripheral blood of patients with gouty arthritis (GA) and to explore its clinical significance. Methods A total of 43 patients with acute gouty arthritis (AGA group), non-acute gouty arthritis (NAGA group) and healthy volunteers (HC group) were enrolled in this study. The levels of IL-8 mRNA and protein in peripheral blood mononuclear cells (PBMCs) were detected by RTPCR and Western blot respectively. The levels of IL-8 in each group were detected by ELISA. Results The expression of IL-8 mRNA in AGA group was higher than that in NAGA group and HC group (all P <0.01), and higher in NAGA group than in HC group (P <0.01). The levels of IL-8 in PBMCs in AGA group were higher than those in NAGA group and HC group (all P <0.01), and were the lowest in HC group. However, there was no significant difference between NAGA group and HC group (P> 0.05). Plasma IL-8 levels in AGA group and NAGA group were significantly higher than HC group (all P <0.01), NAGA group was significantly higher than HC group (P <0.05). Conclusion IL-8 is abnormally expressed in the peripheral blood of patients with GA, suggesting that IL-8 may play an important role in the pathogenesis of GA.