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在N6培养基中添加10~(-4)mol.L~(-1)AgNO_3可促进玉米P9-10自交系幼胚诱导产生Ⅰ型胚性愈伤组织.把这些胚性愈伤组织经高渗处理后作为受体,用基因枪转化含Bt基因的pMG6质粒,通过选择压递增式筛选,得到14个抗性克隆.抗性克隆在3种不同的培养基中共诱导出10个胚状体,经分化得到10个再生植株.经PCR和Southern杂交分析证实有8个再生植株的基因组中整合有Bt基因.ELISA分析结果显示转基因植株每克新鲜叶片中Bt毒蛋白含量在20～200ng之间.
The addition of 10 -4 mol·L -1 AgNO 3 to N6 medium promoted the induction of type I embryogenic callus by maize embryos in P9-10 inbred line. The embryogenic callus After being treated by hypertonic treatment, pMG6 plasmid containing Bt gene was transformed by gene gun, and 14 resistant clones were obtained by selective pressure increasing screening.The resistant clones induced 10 embryoids in 3 different media And 10 regenerated plants were obtained by differentiation.The Bt genes were integrated into the genomes of 8 regenerated plants by PCR and Southern blot analysis.ELISA analysis showed that the content of Bt protein per gram of fresh leaves in transgenic plants was between 20 and 200ng between.