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目的:探讨蜂毒穴位注射抑制肿瘤血管生成作用及其相关机制。方法:建立C57BL/6J Lewis肺癌小鼠模型;隔日测量肿瘤最大径与最小径,计算肿瘤体积,绘制生长曲线;第24天麻醉取材称量瘤质量;解剖显微镜下计数肺上转移结节;采用免疫组化法检测CD34计算肿瘤微血管密度;采用Western Blotting法观察药物对JNK信号通路相关蛋白的影响;采用qPCR法观察HIF-1α和VEGF mRNA的表达。结果:与模型组比较,各治疗组肿瘤体积、瘤质量、肺上转移结节、肿瘤微血管密度、p-JNK、p-c-Jun、HIF-1α、VEGF蛋白及HIF-1α、VEGF mRNA表达均显著降低,且蜂毒穴位注射组的疗效优于穴位组及蜂毒非穴位组。结论:蜂毒穴位注射能抑制肿瘤血管生成,其作用机制与调控JNK信号传导有关。
Objective: To investigate the effect of injection of bee venom on tumor angiogenesis and its related mechanism. Methods: The C57BL / 6J Lewis lung cancer mouse model was established. The maximum diameter and the minimum diameter of the tumor were measured every other day. The tumor volume was calculated and the growth curve was drawn. On the 24th day, the weight of the tumor was weighed and the pulmonary nodules were counted under a dissecting microscope. The expression of HIF-1α and VEGF mRNA was detected by qPCR method. Western blotting was used to observe the effect of drugs on JNK signaling pathway. Results: Compared with the model group, the tumor volume, tumor mass, pulmonary metastasis nodules, tumor microvessel density, p-JNK, pc-Jun, HIF-1α, VEGF protein and HIF- Reduce, and bee venom injection group is better than acupuncture point group and bee venom non-acupuncture group. Conclusion: Injection of bee venom can inhibit tumor angiogenesis and its mechanism is related to the regulation of JNK signal transduction.