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目的 探讨原核表达的可溶性重组人内抑素(rhEndo)对ECV 30 4内皮细胞和原代培养兔主动脉内皮细胞增殖的影响。方法 利用酶标仪进行噻唑蓝 (MTT)法检测 ,同时采用倒置相差显微镜、电子显微镜、流式细胞仪、半胱天冬酶 3活性分析观察该内抑素对碱性成纤维细胞生长因子 (bFGF)刺激的血管内皮细胞增殖的影响。结果 该rhEndo明显抑制ECV 30 4细胞的增殖 ,MultiCycleDNACycle软件分析表明细胞增殖阻滞在G1期 ,流式细胞仪检测发现该内抑素可诱导ECV 30 4细胞凋亡 ,且与半胱天冬酶 3活性增强有关 ,但对原代培养的兔主动脉内皮细胞增殖并未产生明显的作用。结论 内抑素可明显抑制ECV 30 4细胞增殖并诱导其发生凋亡 ,但对原代培养的兔主动脉内皮细胞增殖没有明显的影响 ,这将有利于与血管新生有关的疾病如癌症等的治疗。
Objective To investigate the effect of rhEndo prokaryotic expression on the proliferation of ECV 30 4 endothelial cells and primary cultured rabbit aortic endothelial cells. Methods MTT method was used to detect the content of basic fibroblast growth factor (FST) by MTT assay, inverted phase contrast microscope, electron microscope, flow cytometry and caspase 3 activity assay. bFGF) on the proliferation of vascular endothelial cells. Results The rhEndo significantly inhibited the proliferation of ECV 30 4 cells. The analysis of MultiCycle DN ACycle showed that the cell proliferation arrest was in G1 phase. Flow cytometry showed that endostatin induced the apoptosis of ECV 30 4 cells, 3 activity, but the primary cultured rabbit aortic endothelial cell proliferation did not have a significant effect. Conclusion Endostatin can significantly inhibit the proliferation of ECV-30 4 cells and induce apoptosis of ECV-30 4 cells. However, it has no obvious effect on the proliferation of primary cultured rabbit aortic endothelial cells, which is beneficial to angiogenesis-related diseases such as cancer treatment.