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1 材料和方法 处死小鼠，腹腔内注射 5 mL 冷 Hanks 液，轻揉腹部，抽吸细胞悬液约 4 mL。细胞洗两次后，加含 10％ 小牛血清的 RPMI 1640 培养基 10 mL，接种于 96 孔板中， 每孔 01 mL，37℃ 静置 60 min，使巨噬细胞贴壁。用无血清培养液冲洗培养孔三次，去除未贴壁细胞，剩
1 Materials and Methods The mice were sacrificed and 5 mL of cold Hanks fluid was injected intraperitoneally. The abdomen was gently massaged and the cell suspension was aspirated to about 4 mL. After the cells were washed twice, 10 mL of RPMI 1640 medium containing 10% fetal bovine serum was inoculated into a 96-well plate, and each well was allowed to stand at 01 mL for 60 min at 37 ° C to adhere macrophages. Flush the culture wells with serum-free medium three times to remove unattached cells and leave