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目的:探讨痰咳净粉雾剂对流感病毒感染小鼠肺部炎性损伤的影响及作用机理。方法:以流感病毒甲型鼠肺适应株(FM1)滴鼻感染小鼠建立病毒性肺炎模型,随机分为正常对照组、模型组、痰咳净粉雾剂低、中、高剂量组、利巴韦林组。运用Western-Blot检测感染24 h后NF-κB在肺组织中的表达,阐明药物的调控作用;采用ELISA法检测感染3 d后,肺组织炎性细胞因子TNF-α、IL-1β和IL-6水平的变化;同时观察痰咳净粉雾剂不同剂量对小鼠肺指数及病毒致小鼠死亡率的影响。结果:痰咳净中、高剂量有以下作用:①对感染病毒24 h后的小鼠肺组织中NF-κB表达有下调作用;②能降低小鼠肺组织中TNF-α、IL-1β和IL-6水平(P<0.05);③能降低肺指数;④能改善感染小鼠的临床症状,减少15 d内小鼠的死亡数,并能延长其平均存活时间(P<0.05)。结论:痰咳净粉雾剂可通过调节NF-κB以及炎性细胞因子TNF-α、IL-1β和IL-6的表达水平,从而减轻FM1流感病毒引起的小鼠肺部炎性损伤。
OBJECTIVE: To investigate the effect and mechanism of guanchuangjing powder on inflammatory injury of lung in mice infected with influenza virus. METHODS: Viral pneumonia models were established by intranasally infected mice infected with influenza virus type 1 murine lung admixture (FM1). The models were randomly divided into normal control group, model group, and low-, medium-, and high-dose groups. Bavelin group. Western-Blot was used to detect the expression of NF-κB in lung tissue after infection for 24 h and elucidate the regulation effect of the drug. The inflammatory cytokines TNF-α, IL-1β and IL- were detected by ELISA after 3 days of infection. 6 levels of change; at the same time observe the effects of different doses of Qi cough powder aerosol on mouse lung index and virus-induced mouse mortality. RESULTS: The moderate and high doses of phlegm and cough have the following effects: 1The down-regulation of NF-κB expression in the lung tissue of mice infected with virus for 24 h; 2 The decrease of TNF-α, IL-1β and the lung tissue of mice. IL-6 levels (P <0.05); 3 can reduce the lung index; 4 can improve the clinical symptoms of infected mice, reduce the number of mouse deaths within 15 days, and can extend the average survival time (P <0.05). Conclusion: Cough-cough powder can reduce the inflammatory lesions of mice caused by FM1 influenza virus by regulating the expression of NF-κB and inflammatory cytokines TNF-α, IL-1β and IL-6.